[Histonet] stain for calcium oxalate on paraffin
histology.bc <@t> shaw.ca
Tue Feb 22 19:02:20 CST 2005
The quickest and easiest approach is to view your existing H&E sections
using a polarized light microscope. Calcium oxalate crystals are
strongly birefringest and are impossible to miss. Would I be correct to
assume you have sections of kidney from an ethylene glycol (antifreeze)
The "stains" suggested in some texts are essentially modifications of
von Kossa's silver substitution method for the calcium salts usually
associated with bone or chronic inflammatory reactions. They work, but
are not specific for calcium oxalate as they will also react with any
other insoluble calcium salts that happen to be present.
Give the polarizer a try, I think you will be happy with the results.
Best of luck,
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