[Histonet] her-2 nuclear staining
katri <@t> cogeco.ca
Tue Feb 22 12:02:37 CST 2005
The cases that you find nuclear staining, how long have they been fixed? My
understanding of doing Her-2 is that the tissue has to be fixed minimum 24
hours in 10% NBF (regardless of the size) to avoid false +/- staining.
"Underfixation" is generally bigger problem than "overfixation" and I have
wittnessed false nuclear staining with some antibodies.
Hamilton, Ontario, Canada
----- Original Message -----
From: <dsuer <@t> comcast.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 21, 2005 6:22 PM
Subject: [Histonet] her-2 nuclear staining
> Occasionally we have a her-2 with irregular nuclear staining.It seems to
> be happening on breast needle cores. Could it be over or under fixation?
> If anyone has any information I would greatly appreciate it. D.
> Suermann HT(ASCP)
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