[Histonet] her-2 nuclear staining

Katri Tuomala katri <@t> cogeco.ca
Tue Feb 22 12:02:37 CST 2005

The cases that you find nuclear staining, how long have they been fixed? My 
understanding of doing Her-2 is that the tissue has to be fixed minimum 24 
hours in 10% NBF (regardless of the size) to avoid false +/- staining. 
"Underfixation" is generally bigger problem than "overfixation" and I have 
wittnessed false nuclear staining with some antibodies.

Katri Tuomala
Hamilton, Ontario, Canada

----- Original Message ----- 
From: <dsuer <@t> comcast.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 21, 2005 6:22 PM
Subject: [Histonet] her-2 nuclear staining

> Occasionally we have a her-2 with irregular nuclear staining.It seems to 
> be happening on breast needle cores.  Could it be over or under fixation? 
> If anyone has  any information I would greatly appreciate it.       D. 
> Suermann HT(ASCP)
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