[Histonet] Negative Rabbit Control
Coskran, Timothy M
timothy.m.coskran <@t> pfizer.com
Tue Feb 22 10:19:35 CST 2005
For those using negative Rabbit IgG, how do you determine the dilution of
your negative. Are you matching the IgG concentration of your negative
rabbit IgG to that of the primary antibody at its working dilution? What do
you do when a vendor does not know the IgG concentration of a rabbit
polyclonal primary antibody?
We try to match the isotype and Ig concentration for negative controls when
using monoclonals, but this seems to be a difficult task for rabbit
polyclonal antibodies for which you don't have a starting Ig concentration.
Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
More information about the Histonet