[Histonet] collagen gell autofluorescence

[Pawel,Pasierbek]

Dear All,
One of the users of our microscopy facility is thrying to image cells grown in the collagen gel (DB). After immunostaings she gets a lot of autofluorescence (as I believe) comming from the gel itself - it is visible when imaged with all 3 lasers 488nm, 543nm, 633nm.
Do you have any hint how to get around this problem?
 
All the best from Vienna,
Pawel
 
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Pawel Pasierbek, PhD
Biooptics
IMP (Research Institute of Molecular Pathology)
Dr. Bohr-Gasse 7
1030 Vienna
Austria

Telephone: +43 (1) 79730591
Fax: +43 (1) 7987153
http://www.imp.univie.ac.at <http://www.imp.univie.ac.at>