[Histonet] (no subject)

[Tony Henwood]

I would assume that the formalin would inactivate the RNAses. At least from
literature experience, mRNA has been regularly demonstrated in FFPE tissues.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318







-----Original Message-----
From: Yves Heremans [mailto:herme013 <@t> umn.edu] 
Sent: Tuesday, 22 February 2005 2:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


Dear All,
I was wondering whether 4% formalin used for fixation before RNA in 
situ hybridization has to made up RNAse-free or does the fact that it 
is a fixative renders it de facto RNAse-free ?
Yves


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