[Histonet] Oil Red O

[Mary North]

I cover initially with Crystal Mount and let it dry at room temp.  After
drying, it can be dipped into xylene and coverslipped with resinous
medium.  
Mary North
OHSU Neuromuscular Lab
Portland, OR

>>> "Till, Renee" <TillRenee <@t> uams.edu> 02/17/05 11:22 AM >>>
Hello. We have been doing some Oil Red O stains on cryosections of
aorta
and have been having problems with the hematoxylin leaching out during
mounting. We have tried several different aqueous mounting medias and
none have corrected the problem. Could it be the tissue or maybe the
type of hematoxylin? Another lab has been doing the stain on liver and
hasn't had any problems.

 

Thanks,

Renee' Till


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