[Histonet] antigen retrieval question

[Kelly D Mcqueeney]

I have never seen a problem when using antibodies that require an 
overnight incubation.

Jim Staruk wrote:

>We tried that once and the control slide (along with the research slides)
>were completely negative.  Not sure if it was a fluke, but we never tried it
>again!  Now everything is done on the same day.
>
>Jim
>
>______________________
>    Jim Staruk
>Mass Histology Service
>www.masshistology.com
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
>Sent: Thursday, February 17, 2005 3:33 PM
>To: ihcrg <@t> neo.agsci.colostate.edu
>Cc: histonet <@t> pathology.swmed.edu
>Subject: [Histonet] antigen retrieval question
>
>I have heard that it is not a good idea to do HIER one day and do the IHC
>the next?  Is this so?  I heard that the unmasking could actually remask if
>left sitting a long time before doing the staining.  I have a protocol that
>requires a 3 hr. water bath incubation for AR and it would be more
>convienent if I could do that one day and do the rest of the procedure the
>next.
>Patsy
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