[Histonet] Re: B5 fixed tissue

[Richard Cartun]

Dear Melanie & Histonet:

I work with 5 hematopathologists who I have a great deal of respect for
and I can tell you that our service is "First Class" all the way.  We
eliminated B5 about 1.5 years ago and, yes, they were very concerned
about the effect it would have on morphology.  We tried several of the
B5 substitutes, but eventually we ended up fixing all bone marrows in
formalin.  Their "only" complaint is that the CD15 (Leu-M1)
immunoperoxidase stain is weaker on formalin-fixed tissue than with B5. 
I can also tell you that immunoreactivity for a wide range of proteins
is stronger and more reproducible with formalin than with B5.  I see no
reason to continue using B5.  Hopefully, it will be eliminated from all
laboratories in the near future.

Richard 

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Trivett, Melanie" <Melanie.Trivett <@t> petermac.org> 02/16/05 05:51PM
>>>
we do for our bone marrow trephines.  I would love to eliminate it for
handling problems, disposal issues and IHC consistency.  Our
haematologist will not hear of it.  I would love to hear any good
arguments that you have in addition!  He still thinks there is nothing
as good as B5 for the morphology of the trephines.

Melanie

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
Cartun
Sent: Thursday, 17 February 2005 8:00 AM
To: histonet <@t> lists.utsouthwestern.edu; sluhisto <@t> yahoo.com 
Subject: Re: [Histonet] cd5 and cd23 on B5 fixed tissue


How many labs still use B5?  We should work towards eliminating it
from
our environment.

Richard 

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Histology SLU <sluhisto <@t> yahoo.com> 02/16/05 02:23PM >>>
Hello All:
 
Is anyone doing these two antibodies on B5 fixed tissue with good
results?  If you are, and would be willing to share your secrets, any
info would be appreciated.  Both of the vendors and a couple of others
have not used these antibodies with B5 fixation as part of their QC
procedures and can not help.  Thanks, in advance.
 
Susan
 

		
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