[Histonet] Antibody Purification for Quantum Dots experiment

[RCHIOVETTI <@t> aol.com]

In a message dated 2/16/2005 11:45:13 AM US Mountain Standard Time, 
skouko <@t> po-box.mcgill.ca writes:

> How do I purify my antibody
> without losing half the content (as would happen if I used dialysis). Any
> ideas?
> 

Hi Sophia,

You could probably get a fairly good yield by using affinity purification 
with either a Protein G or Protein A - Sepharose column.  The exact protocol 
would depend on the origin of the antibody, IgA / IgM, etc.  Protein G seems to be 
used more frequently than Protein A.

For background, see for example:
<http://iacuc.cwru.edu/policy/mabs.html#pure>

Good luck!

Cheers,

Bob Chiovetti