[Histonet] HIER and EIER

Sebree Linda A. la.sebree <@t> hosp.wisc.edu
Wed Feb 16 13:27:21 CST 2005

We've always done our HIER before the EIER but mainly because that's how our instruments proceed.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
bliven.laura <@t> marshfieldclinic.org
Sent: Wednesday, February 16, 2005 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HIER and EIER

When used in combination, is it better to use HIER (Heat Induced Epitope Retrieval) first or EIER (Enzyme Induced Epitope Retrieval) first.  Or does it depend upon the antigen of interest?

Any references?

Laura Bliven

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list