[Histonet] Antibody Purification for Quantum Dots experiment

skouko <@t> po-box.mcgill.ca skouko <@t> po-box.mcgill.ca
Wed Feb 16 12:42:41 CST 2005

Hi guys,
I want to use the Quantum Dots conjugation kit to label my primary antibody for
an immunohistochemistry experiment. The QD protocol requires the antibody to be
ascites and protein free. Unfortunately my antibody has ascites in it. I've
already performed a "www.biocompare.com" search and there is no equivalent
antibody on the market that is ascites free. How do I purify my antibody
without losing half the content (as would happen if I used dialysis). Any
Sophia Koukoui
Behavioural Neuroscience
McGill University.

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