[Histonet] RE: Problems with floating sections

Prior, Andrew Andrew.Prior <@t> Smith-Nephew.com
Wed Feb 16 03:43:00 CST 2005

We had some success using 2N HCl at 37deg C for 30 minutes. This was enough
to uncover the epitope and sections stayed on slides.
If you are cutting more sections try using pre-coated (poly-L?) or charged
slides (Superfrost Plus) as they help sections stick during IHC 


Andrew Prior
Smith & Nephew Research Centre
York Science Park

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