[Histonet] B-Plus Fixative

Browning Deb browning <@t> HHSC.CA
Tue Feb 15 07:45:20 CST 2005

We are currently using B5 fixative for bone marrows, and as much as we don't
like using it, and it gives problems with some of the antibodies, after
extensive testing of B5 substitutes, I found them all inferior to B5.  The
only fixative that gave consistent staining with all antibodies was 10% NBF,
but only after 24 hours fixation, and this time limitation was not
acceptable for some clinical cases.  Our biggest problem with 10% NBF was
producing a H&E section that demonstrated the same crispness of nuclear
detail that is achieved with B5.

-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Friday, February 11, 2005 4:30 PM
To: Histonet
Subject: [Histonet] B-Plus Fixative

My pathologists are unhappy with B-Plus fixed bone marrows, especially
immunos. They have pulled previous cases on patient that were fixed in B5
and of couse see quite a difference side by side. Does anyone else hear this
Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

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