[Histonet] B-Plus Fixative

[Browning Deb]

We are currently using B5 fixative for bone marrows, and as much as we don't
like using it, and it gives problems with some of the antibodies, after
extensive testing of B5 substitutes, I found them all inferior to B5.  The
only fixative that gave consistent staining with all antibodies was 10% NBF,
but only after 24 hours fixation, and this time limitation was not
acceptable for some clinical cases.  Our biggest problem with 10% NBF was
producing a H&E section that demonstrated the same crispness of nuclear
detail that is achieved with B5.

-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Friday, February 11, 2005 4:30 PM
To: Histonet
Subject: [Histonet] B-Plus Fixative


My pathologists are unhappy with B-Plus fixed bone marrows, especially
immunos. They have pulled previous cases on patient that were fixed in B5
and of couse see quite a difference side by side. Does anyone else hear this
complaint?
 
Thanks,
Joyce
 
Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
 


Confidentiality Notice ** The information contained in this message may be
privileged and is confidential information intended for the use of the
addressee listed above. If you are neither the intended recipient nor the
employee or agent responsible for delivering this message to the intended
recipient, you are hereby notified that any disclosure, copying,
distribution or the taking of any action in reliance on the contents of this
information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer.
Thank you. Saint Josephs Health System, Inc.


This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed.  Therefore, this
information should be considered strictly confidential.  If you have
received this email in error, please notify the sender immediately via a
return email for further direction. Thank you for your assistance.