[Histonet] Dako Autostainer

[Hopkins, Karen]

Hi We have had a problem with our IHC autostainer and I was wondering if
anyone else has experienced the same problem.  We had a Dako Autostainer
and over time (about three years) found that we had intermittent
problems with slides not being stained.  It started with slides in the
first and second slide position (the positive control was not staining)
when we had a large run but eventually we noticed it at other positions,
spiratically.  We have another instrument now, since Nov and the other
day experienced the same problem.  We ran three sets of known controls
(we send one to each of the facilities we stain for) and one of the
three was positive, the other two were not.  We were using a RTU ER
antibody. ????    Also, the spec sheet from Dako states we should run
the ER with Env or Lsab2.  We get better results with ENV+.    The stain
with ENV is extremely pale.  Any comments or suggestions?