[Histonet] Elastin Stain for GMA/MMA (fwd)

[Patsy Ruegg]

I have done Pentachrome stain for elastic fibers on rat pulmonary arteries
with good results, I use the Pentachrome staining kit from Polyscientific.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Y. Wang
Sent: Thursday, February 10, 2005 2:16 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Elastin Stain for GMA/MMA (fwd)

Hello Catherine,

When we did our elastin staining of rat and tissue enineered arteries we
used 
Hart's mofified method counterstained with tartrazine, as recommended by
Barry 
Starcher (we tried an elasting staining kit which didn't work veyr well for 
us). We only stained arteries alone (no stents) and we are not experts in 
histology. I'm sure someone else on the histonet would be a better source
for a 
protocol. However, we do have the protocol we used if you would like it.

Best regards
Yak-Nam Wang


> Hello Everyone,
> 
> 
> 
> I have been trying to find an elastin stain for GMA and MMA stented
arteries. 
> Does anyone know of a procedure or publication that may help?
> 
> 
> 
> Thanks,
> 
> Cathy
> 
> 
> 
> Catherine L. Scott, B.A., HT (ASCP), ALAT
> 
> Associate Director, Pathology Services
> 
> 54943 North Main Street
> 
> Mattawan, Michigan 49071-9366 USA
> 
> Telephone: 269.668.3336 ext. 1218
> 
> Fax: 269.668.4151
> 
> email: catherine.scott <@t> mpiresearch.com
> 
> 
> 
>
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