[BULK] - [Histonet] Water bath temperature

Rebecca Barnhart rbarnhart <@t> summithealth.org
Fri Feb 11 15:05:30 CST 2005

We do not use alcohol in the water bath.  We use a product called Halt
and I tested it at 45 with and with out Halt, both had polka dots.  

>>> "Fred Underwood" <funderwood <@t> mcohio.org> 2/11/2005 3:43:58 PM >>>
Hi Rebecca,

This is just a dart at the old dart board.  Could you be getting
bubbles trapped under your sections?  This is also more likely to
if you're using alcohol in the water bath. 

...ahhh, darts and a cold one on a Friday.  Anyone care to join me?


>>> "Rebecca Barnhart" <RBARNHART <@t> summithealth.org> 02/11/05 02:24PM
We were having a problem that the tissue would not take up the
hematoxlyin but would take up the eosin but only in some areas, so it
looked like polka dots.  We started one by one changing solution
to figure out where it was coming from.  We had all fresh solutions
still had polka dots.  I tried the only other thing left to change, I
lowered the temperature of the water bath from 45 to 38 and this
 We currently are using ParaPlast Xtra with a melting point of 52.  We
have all tried figure out why the temperature in the water bath will
make the difference.  Several times I have cut the same tissue at
different temperatures ranging from 38 - 45 and every time there are
polka dots at the higher temperatures.  Does anyone have any idea why
this would be?  

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