[Histonet] Abandoned Warthin-Starry protocol in favor of Steiner

[Gayle Callis]

Dear all,

After dinking with WS, we went with Steiner (in house made reagents) and 
used a superb protocol from Peggy Wenk's laboratory.  We could do several 
racks of slides in a day, mass production with some organization, but there 
were no headaches with her method - it never failed to work well.  Key was 
timing and watching that control develop.  I fully agree, a good positive 
control is needed. We are forever grateful to Peggy on this one.

At 08:08 AM 2/11/2005, you wrote:
>What particular protocol are you using?  Lab made or
>commercial?  Is it possible that your control tissue
>has been depleted?
>Back when I still in clinical, no one else would do
>them and I always agreed with the saying that they
>were 'worthless and sorry'.  Once in a very, very blue
>moon I got results that were worth something.
>
>Vikki Baker
>Mt. Sinai School of Medicine
>New York, NY 10029
>
>
>--- "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV> wrote:
>
> > Good W-S?  Isn't that an oxymoron?
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> > On Behalf Of Thomas
> > Wood
> > Sent: Friday, February 11, 2005 9:54 AM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Warthin-Starry protocol needed
> >
> >
> > Can anyone give me a good Warthin-Starry protocol
> > for spirochetes, ours
> > no longer does the job.
> >
> > Tom Wood BS HT
> > Michigan State University
> > Veterinary Medicine
> >
> >
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>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)