[Histonet] Region VII/CSH Meeting

Fri Feb 4 14:58:05 CST 2005

This is posted as an invitation to attendees and vendors to join Region VII
and the Colorado Society for Histotechnology at our Symposium Convention in
April 2005. Please help us get the word out.

Patsy Ruegg

2005 Colorado Society of Histotechnology & NSH Region VII Meeting

( <outbind://11/www.coloradohisto.org/2005> www.coloradohisto.org/2005)

Dates: April 22 & 23, 2005

Location:

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Beaver Run Resort

620 Village Rd. 

Breckenridge, CO 80424-2115

Ph: (800) 525-2253 

(970) 453-6000

Fx: (970) 453-4284

 <outbind://11/www.beaverrun.com> www.beaverrun.com 

Registration: 

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Friday: 12:00 - 1:00

Saturday: 7:00 - 8:00 

Exhibitors: 

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Friday: 9:30 - 6:00

Saturday: 9:30 - 5:00 

Schedule: Friday, April 23rd

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12:00 - 1:00 Registration

1:00 - 2:30 Class 1-A or 1-B

2:30 - 3:00 Break

3:00 - 4:30 Class 2-A or 2-B

4:30 - 6:00 Social 

Saturday, April 24th

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7:00 - 8:00 Registration and Breakfast

8:00 - 9:30 Class 3-A or 3-B

9:30 - 10:00 Break

10:00 -11:30 Class 4-A or 4-C

11:30 - 1:00 Lunch and CSH Business Meeting 1:00 - 2:30 Class 5-A or 5-B
2:30 - 3:00 Break 3:00 - 4:30 Class 6-A or 6-B 4:30 - 5:00 Vendo drawing

Deadlines:

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Hotel Reservations - March 8, 2005

CSH Pre-registration - April 2, 2005

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PROGRAM 

Note: Some of the speaker abstracts were not available at the time of this
posting. However, all abstracts are being posted on the CSH website as they
become available. We apologize for any inconvenience. 

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SESSION 1: FRIDAY AFTERNOON, APRIL 22, 2005 

1:00 PM - 2:30 PM, Presentations 

1-A. The History of Automation in Histology Mr. H. Skip Brown, B.A., HT

(ASCP) Lab Management Consultants - St. Louis, MO 

Abstract Pending

CEU credit hours: 1.5 

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1-B. Update for Markers in Breast Cancer Dr. Meenakshi Singh, M.D. Associate
Director of Surgical Pathology, UCHSC - Aurora, CO 

This presentation will cover the clinical relevance of estrogen receptor,
progesterone receptor and Her 2neu analysis by immunohistochemistry, image
analysis and fluorescent in situ hybridization studies in invasive breast
cancer. The expression profile of a patient's tumor for these markers is
used in tailoring treatment decisions and assessing prognosis for individual
patients. Recently the scope of ER/PR IHC has been expanded to also being
incorporate these into the clinical work up of cases of non-invasive breast
cancer (ductal carcinoma in situ). The potential uses of newer markers in
breast cancer and metastatic breast cancer will also be discussed; these
shall include clinical research done in Dr. Singh's laboratory at the
University of Colorado Health Sciences Center in recent years.

CEU credit hours: 1.5 

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2:30 PM - 3:00 PM, Break (Breckenridge Ballroom) 

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3:00 PM - 4:30 PM, Presentations 

2-A. The Challenges of Change

Mr. H. Skip Brown, B.A., HT (ASCP) Lab Management Consultants - St. Louis,
MO 

Abstract Pending

CEU credit hours: 1.5 

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2-B. Changes in the QIHC

Ms. Patsy Ruegg, HT(ASCP) QIHC IHCtech, FitzSimmons Bioscience Park -
Aurora, CO.,  <outbind://11/www.ihctech.net> www.ihctech.net 

This presentation can help prepare potential examinees for the new written
format for the QIHC exam by going over the projects required in the past and
their application to the written questions anticipated on the new exam. The
subject categories asked about on this written exam will be covered in this
presentation. A list of IHC theory books and handbooks to use to prepare for
the exam will be provided.

CEU credit hours: 1.5 

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SESSION 2: SATURDAY MORNING, APRIL 23, 2005
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8:00 AM - 9:30 AM, Presentations 

3-A. Contrasting Sharp and Blunt Force Trauma Dr. Stephen Cina, M.D. 

Weld County Coroner

Pathologist, Mckee Medical Center - Loveland, CO 

Following this presentation, the participant will be able to: 

1. Discriminate between sharp force and blunt force injuries. 

2. Recognize the significance of "pattern injuries." 

3. Associate the types of injuries caused by various weapons types. 

A cutaneous injury may be the result of blunt or sharp force trauma. Even to
the trained eye, it may be difficult to discriminate between lacerations,
cuts, stabs or gunshot wounds. This presentation will focus on the
diagnostic features of blunt force and sharp force injuries. Mechanisms of
these types of injuries will be discussed. Due to the nature of the topic,
this presentation will utilize many graphic images to illustrate salient
points. 

CEU credit hours: 1.5 

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3-B. Localization of Mycobacterium tuberculosis in diseased tissue utilizing
in situ hybridization in combination with immunohistochemistry Ms. Liz
Chlipala, B.S., HTL (ASCP) QIHC, Premier Lab, LLC. - Boulder, CO,
<outbind://11/www.premierhistology.com> www.premierhistology.com Ms. Allison
L. St. Amand, M.S., University of Colorado at Boulder 

Diagnosis of tuberculosis generally relies on a combination of acid fast
staining of histological specimens, and culture methods. AFB staining is
unable to speciate different mycobacteria. In addition mycobacterial acid
fastness can be partly or completely lost at some stages of growth of the
organisms. Alternatively in situ hybridization can be used to detect
bacterial distribution and morphology in the context of histopathology of
the tissue. In situ hybridization utilizing 16S ribosomal RNA
oligonulceotide probes allows for rapid speciation of mycobacteria. This
technique used in combination with immunohistochemistry allows for better
understanding of the disease process. This lecture will demonstrate the use
of ISH, IHC, and IF in animal models of Mycobacterium tuberculosis
infections.

CEU credit hours: 1.5 

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9:30 AM - 10:00 AM, Break (Breckenridge Ballroom) 

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SESSION 2: SATURDAY MORNING, APRIL 23, 2005
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10:00 AM - 11:30 AM, Presentations 

4-A. Theory and Practice of Microwave Technology Mr. H. Skip Brown, B.A., HT

(ASCP) Lab Management Consultants - St. Louis, MO 

Abstract Pending

CEU credit hours: 1.5 

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4-B. The Human Genome Project

Ms. Donna Lawson, HT (ASCP) QIHC Technical Trainer, Ventana Medical Systems

- Tuscon, AZ,  <outbind://11/www.ventanamed.com> www.ventanamed.com 

The Human Genome Project was officially started in 1990; the involvement
became a world wide project of scientists and organizations. The goal was to
generate a high-quality reference DNA sequence for the human genome's 3
billion base pairs and to identify all human genes. The project completed in
2003. Even though it was a federally funded project there was great effort
to make the information discovered available to the private sector. Grants
were funded for innovative research which in turn created a multibillion
dollar biotechnology industry. What does this all mean to the medical
profession? Because of the project a new world of drug discovery, genetic
testing, and bioethics were created. This workshop will cover the inception
of the Project and the terminology of genetics in a simple format. It will
also discuss the areas the project has touch in medicine, agriculture and
industry. The questions of bioethics that arose during the project will be
covered along with some possible answers. 

CEU credit hours: 1.5 

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11:30 AM - 1:00 PM Lunch & CSH Business Meeting (Breckenridge Ballroom) 

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SESSION 3: SATURDAY AFTERNOON, APRIL 23, 2005
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1:00 PM - 2:30 PM, Presentations 

5-A. Great Cases from Small Places (I)

Dr. Thomas Haas, D.O. Pathologist, Mercy Health System - Janesville, WI 

In many hospitals, there is no communication or connection between the work
done by histotechnologists, the interpretations and recommendations made by
pathologists, and the final diagnosis and treatment of the patient by
clinicians. Oftentimes, the histotechnologist is not encouraged to attend
"Tumor Boards" or other peer-review situations involving patient care. This
can be frustrating to those involved in the "behind the scenes" work of the
laboratory, and can tend to squelch understanding, interest, and learning on
the part of the histotechnologist, when no feedback or integration of "why"

certain stains and investigative laboratory techniques were ordered. This
seminar will explore a number of different pathology cases and their final
diagnoses, beginning with patient information, progressing through the
reasoning behind ordering special stains, immunohistochemistry, flow
cytometry, and other pertinent laboratory methods, with an explanation of
the disease process, diagnostic methods, and final treatment of the patient.

These cases range from those seen everyday in the hospital pathology
laboratory, to those requiring outside expert interpretation. 

CEU credit hours: 1.5 

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5-B. Laser Capture Microdissection (LCM) - HT Perspective Ms. Ann Bennett
Lossing, Field Application Scientist, Arcturus, Inc. - Mountain View, CA,
<outbind://11/www.arctur.com> www.arctur.com 

Gene Expression profiling of specific cell types in both frozen and
formalin-fixed breast cancer biopsy samples. 

Microarrays are valuable tools for studying normal and induced variations in
gene expression. Microgram amounts of total RNA are required for target
preparation for most microarray platforms. Consequently, whole tissue
biopsies are typically used for these studies. However, distinct differences
have been shown between gene expression data obtained from whole tissue
biopsies, which are essentially mixed cell populations, and that obtained
from homogenous populations of cells. Microdissection enables the profiling
of native expression levels of thousands of genes, in a selected cell
population. In this presentation we will show how microdissection, combined
with RNA amplification to produce the amounts of aRNA needed for microarray
analysis, have allowed us to generate expression profiles in specific cell
populations obtained from breast cancer biopsy samples. As a result of
recent technological advances, we are able to isolate RNA from both frozen
and formalin fixed tissue samples, and used this RNA to obtain expression
profiles. These highly reproducible expression profiles have been used to
generate molecular signatures for different stages of breast cancer using
frozen biopsy tissues and microarray analysis. Our presentation will focus
on the importance of developing histological methods for providing excellent
visualization of cell types, while at the same time, preserving preserve RNA
quality. 

Topics to be discussed:- Laser Capture Microdissection Technology
Microgenomics Reagent Systems Paradise T Formalin-Fixed Tissue - Expression
Analysis System Application Review - Arcturus LCM-based Publications (over
550 available to date!) 

CEU credit hours: 1.5 

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2:30 PM - 3:00 PM Break (Breckenridge Ballroom) 

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3:00 PM - 4:30 PM, Presentations 

6-A. Great Cases from Small Places (II) Dr. Thomas Haas, D.O. Pathologist,
Mercy Health System - Janesville, WI 

See presentation 5-A for abstract

CEU credit hours: 1.5 

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6-B. Dewax, Rehydration, and Antigen Retrieval the Easy Way Ms. Patricia
Piatti & Mr. Allen Younger Biogenex, San Ramon, CA 

How do you conduct Dewax, Rehydration, and Antigen Retrieval for your
formalin-fixed, paraffin-embedded tissue sections? Do you baby-sit every
tedious step of toxic xylene and alcohol dewax and rehydration pretreatment?

Do you have difficulty in preserving tissue sample morphology and obtaining
consistent results when you using traditional pressure cookers, water baths,
and microwaves for antigen retrieval? Do you need a lot of guess work and
training? Now, with BioGenex's EZ Retrieval System, the sample pretreatment
has never been as easy as today. EZ RetrieverT allows you to shorten
multiple steps of traditional tissue sample pretreatment from 85min to less
than 30 mins. With a throughput as high as 96 slides/run, you may even save
more than 4 hours of work and you can almost walk away from the whole
process. With the ease of use digital interface and temperature probe, you
can standardize the procedure with little training. By using eco-friendly
ZEN-AR solutions that have been optimized for each antibody, you can
preserves the tissue morphology and gain high quality and reproducible
results. To learn more about the ZEN Retrieval System, please join the ZEN
Retrieval System work shop that will be held in the following locations or
visit our website at  <outbind://11/www.biogenex.com> www.biogenex.com. 

CEU credit hours: 1.5 

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4:30 PM - 5:00 PM, Vendo drawing (Breckenridge Ballroom) 

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Online registration and additonal information regarding the meeting can be
found at  <outbind://11/www.coloradohisto.org/2005>
www.coloradohisto.org/2005