[Histonet] Same Host Ab flour problems

[Coskran, Timothy M]

If you know the Ig concentration of the telomerase mouse monoclonal, you
could try to biotinylate it using the Dako ARK kit.  Then follow this with
streptavidin Alexa 488 from Molecular Probes.  This technique was presented
by Chris van der Loos at an NSH a while back.  The ARK kit provides an easy
way to biotinylate a mouse antibody so that you can base the detection of
this antibody on biotin and then use an anti-mouse IgG secondary (HRP,
fluor, etc) for the second mouse antibody.  You may have to titrate the
telomerase antibody.  In my experience, you have to increase your primary
antibody concentration when using the ARK kit.

Good Luck

Tim Coskran
Pfizer



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