[Histonet] Nuclear staining

Tony Henwood AnthonyH <@t> chw.edu.au
Wed Feb 2 17:32:06 CST 2005


Try treating the cut sections with 1% Periodic acid for 10-15 minutes and
then rinse in dilute Lithium Carbonate for 10 minutes prior to haematoxylin
staining. You might also double your Hx staining time and decrease the eosin
staining time to give you better results.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Bernardo Vargas-Angel [mailto:vargasb <@t> nova.edu] 
Sent: Thursday, 3 February 2005 4:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear staining

I am working with scleractinian corals and I am experiencing problems with
nuclear staining (H&E), particularly one species: Montastraea cavernosa.
Initially, we attributed lack of basophilia to overdecalcification, however
after decreasing the acid concentration from 5% to 2.5% and being really
careful to assess the decal endpoint, we still have very weak nuclear
staining (for the aforementioned species). Other species of corals that I
have been working with have responded quite favorably to the decreased acid
concentration (increased basophilia); however, not M. cavernosa. Has any out
there encountered similar problems with other kinds of tissues? Have any of
you observed differential tissue sensitivity to acid decal? 
Any insight would be greatly appreciated. 
Best and many thanks in advance.

Bernardo Vargas-Angel  Ph.D.
Research Scientist
National Coral Reef Institute
Nova Southeastern University
8000 N Ocean Drive, Dania Beach, FL, 33004
Voice +(954) 262-3677
Fax +(954) 262-4027

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