Reference to Gayle on RE: [Histonet] another background / non-specific staining problem

SMITH,REBEKAH FELICIA rockbeki <@t> ufl.edu
Wed Feb 2 16:25:12 CST 2005


Sorry 'bout that, I guess I got people mixed up.
On Wed Feb 02 10:28:28 EST 2005, Gayle Callis 
<gcallis <@t> montana.edu> wrote:

> Rebekah,
> 
> Sorry folks but this is not a recommendation from me.
> 
> I have never done this!
> 
> At 08:42 PM 2/1/2005, you wrote:
>> I had a similar problem when staining non-perfused sheep 
>> placenta with eNOS and someone (I think it was Gayle) on 
>> Histonet suggest putting my slides in 1% NaOH (the other 99 
>> being ethanol) solution. I've been putting my slides in 1% NaOH 
>> for 15 min after the peroxide and it has worked well.
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> 
> 
> 



--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"





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