[Histonet] Nuclear staining

[Bernardo Vargas-Angel]

I am working with scleractinian corals and I am experiencing problems with
nuclear staining (H&E), particularly one species: Montastraea cavernosa.
Initially, we attributed lack of basophilia to overdecalcification, however
after decreasing the acid concentration from 5% to 2.5% and being really
careful to assess the decal endpoint, we still have very weak nuclear
staining (for the aforementioned species). Other species of corals that I
have been working with have responded quite favorably to the decreased acid
concentration (increased basophilia); however, not M. cavernosa. Has any out
there encountered similar problems with other kinds of tissues? Have any of
you observed differential tissue sensitivity to acid decal? 
 
Any insight would be greatly appreciated. 
 
Best and many thanks in advance.
 

Bernardo Vargas-Angel  Ph.D.
Research Scientist
National Coral Reef Institute
Nova Southeastern University
8000 N Ocean Drive, Dania Beach, FL, 33004
Voice +(954) 262-3677
Fax +(954) 262-4027
http://www.nova.edu/ocean/vargas-angel/index.html