[Histonet] IL-6

[Elizabeth Chlipala]

Naira

I have stained for IL-6 on formalin fixed, formic acid decalcifed rat
joints.  I use the antibody from Abcam ab6672.  I used proteinase K
digestion with envision +.  This antibody is directed against human
IL-6.  My dilution was 1:300 for 30 minutes at RT.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Margaryan, Naira
Sent: Wednesday, February 02, 2005 9:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IL-6

Hi everyone,

 

Is anybody have been tried to use IL-6 for the FFPE Breast tissue
(normal and carcinoma)? If yes, what protocol did you use?

I have a Monoclonal anti-human IL-6 Ab from R&D (cat#: MAB206) and I
tried to IHC with DAB staining on the breast tissue. Unfortunately, I
didn't get any staining.

 

Is anybody can send me FFPE tonsil tissue as a control?

 

Thanks,

Naira

 

Naira V. Margaryan, Ph.D, D.V.M.

Research Associate II

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-880-4000/5-6740

Fax: 773-755-6594

nmargaryan <@t> childrensmemorial.org
<mailto:nmargaryan <@t> childrensmemorial.org> 

 

For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314

 



The contents of this e-mail message and any attachments are private and
confidential communications intended solely for the addressee(s) named
in this message. If you are not the intended recipient of this message,
please 1) immediately notify the sender by reply e-mail and then delete
this message and its attachments and 2) do not read, use, distribute
disclose or copy this message and/or any attachments. 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet