[Histonet] Problem with mucicarmine stain

[Roxanne Soto]

        A few more questions before we can help.....
  a.. Are you using tap water or distilled to make the working mucicarmine? 
  b.. How long are you leaving it in the mucicarmine? 
  c.. Is this done at room temp? 
  d.. Do you use hematoxylin?
Roxanne
  ----- Original Message ----- 
  From: Chung, Luong<mailto:lchung <@t> ppmh.org> 
  To: Histonet (E-mail)<mailto:histonet <@t> lists.utsouthwestern.edu> 
  Sent: Tuesday, February 01, 2005 2:04 PM
  Subject: [Histonet] Problem with mucicarmine stain


  All,

  When we do mucicarmine (Richar/Allen pre-made) according to our protocol with dilution of one to four.  We counterstain with tretrazine.
  We use appendix as a control and the stain turn out to be very pale.  Is there any remedy for this?
  It used to work very well, but not lately.

  Anne

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