[Histonet] Excessive shrinkage[Scanned]

[Kemlo Rogerson]

If it normally doesn't happen then what makes you think it was the Bouins
and is there anything else you changed. Bouins is alcoholic Picric Acid
isn't it? I assume it's an additive coagulant fixative and if you leave
tissue in it too long it goes rock hard. Dimly in my memory we used to wash
out the picric acid but I don't understand why we put it in, in the first
place. Has your processing changed? Are you leaving the tissue in Bouins too
long?

-----Original Message-----
From: Kim O'Sullivan [mailto:Kim.Osullivan <@t> med.monash.edu.au] 
Sent: 01 February 2005 06:41
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Excessive shrinkage[Scanned]

Hi all,

Can anyone tell me what may cause bouins fixed tissue (in this case mice
kidneys's) to shrink after tissue processing (6 hour).This normally does not
happen.  

ta 

Kim

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet