[Histonet] CY2/CY3 vs Alexa488/546-555

[IGOR NASONKIN]

Andrea,

i use both Alexa 546/488 and Cy2/3. The only crucial difference i found so far is that Cy2 does not work with Vectashield mounting medium, while A's naturally do. The choice may also depend on the filter setup of your fluorescent scope. 

Dr. Igor O. Nasonkin Ph.D.
Postdoctoral Fellow
Pathology/Neuropathology
Johns Hopkins University
Ross Research Bldg Rm 563
720 Rutland Ave
Baltimore MD 21205
tel: 617-388-4104
Igor.Nasonkin <@t> jhmi.edu

----- Original Message -----
From: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
Date: Wednesday, December 21, 2005 7:40 pm
Subject: [Histonet] CY2/CY3 vs Alexa488/546-555

> Has anyone done a SIDE-BY-SIDE comparison of Cy2 or Cy3 as compared 
> with Alexa 488 or Alexa 546/555? I am ideally looking for 
> experimental results not anecdotal information if possible .... I 
> know we have all heard that Alexas are the best and this is 
> certainly 
> true as compared to FITC. But are they better compared to CY2 and 
> CY3 
> from Jackson La
bs?
> 
> I ask b/c I love Jackson Labs secondaries and they don't sell Alexa 
> conjugates :( I use Alexa Streptavidin from Molecular Probes but am 
> not such a fan of their secondaries for TISSUE staining (they give 
> me 
> some strange artifacts sometimes although they work great for cell 
> culture work). I want to keep ordering Jackson ImmunoResearch 
> quality 
> secondaries  - they are just SO SPECIFIC for mouse work but they 
> only 
> sell CY2 and CY3 (which have worked for me thus far).
> 
> Decisions, decisions .... Comments, opinions, suggestions?
> 
> THANKS!!
> Andrea
> -- 
> 
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