[Histonet] Mouse liver tissue processing

[Ingles Claire]

Try a 5-10% aqueous ammonium hydroxide. We used to put this in a small sandwich size tupperware container on top of our ice trays and soaked the blocks in them after facing for about 10-15 minutes before taking sections. They cut great after that, especially small GI and liver bx's that were processed with the longer protocols with our big tissues. You may have to resoak after taking a few sections, but there you go. I usually gave a quick swish in regular cold water to get the excess amm. hydroxide off the block before sectioning.
Claire Ingles
UW Mohs Clinic
Madison WI

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of HSRL
Sent: Wed 12/21/2005 10:02 AM
To: 'Luis Chiriboga'; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Mouse liver tissue processing



Luis,

Have a tried keeping them on a WET ice for 45 minutes to an hour?  If
you have tried it and it doesn't work, it is a processing problem that
you must rectify.

-Tom

Tom Galati
Laboratory Director
HSRL, Inc.- A GLP Compliant Contract Laboratory
5930 Main Street
Mount Jackson, Virginia  22842
540.477.4440
540.477.4448
tomgalati <@t> hsrl.org
www.hsrl.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Luis
Chiriboga
Sent: Wednesday, December 21, 2005 9:54 AM
To: Histonet
Subject: [Histonet] Mouse liver tissue processing

Hi everyone
We are having a real serious problem getting good sections from mouse
livers.  The tissue curls and shreds out of the block as soon as it
comes in
contact with the knife, end up with a section that has the exact outline
of
the tissue but no tissue. The tissue consistency could best be described
as
"dry?" Interestingly, it is only the normal livers that have this
problem.
We have tried sectioning thick, thin, no soak, cold soak......but out of
ideas. Anyone have any suggestions? Unfortunately we have no control
over
the tissue processing, but perhaps can convince to change.
Thanks Luis

Happy Holidays to all!!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet