[Histonet] Mouse liver tissue processing
Luis.Chiriboga <@t> med.nyu.edu
Wed Dec 21 08:54:28 CST 2005
We are having a real serious problem getting good sections from mouse
livers. The tissue curls and shreds out of the block as soon as it comes in
contact with the knife, end up with a section that has the exact outline of
the tissue but no tissue. The tissue consistency could best be described as
"dry?" Interestingly, it is only the normal livers that have this problem.
We have tried sectioning thick, thin, no soak, cold soak......but out of
ideas. Anyone have any suggestions? Unfortunately we have no control over
the tissue processing, but perhaps can convince to change.
Happy Holidays to all!!
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