[Histonet] 17. fluorescence immunohistochemistry

[John Kiernan]

When I was a student in Birmingham, England 
(that's the Birmingham with the silent h), the 
immunology and serology lecturer told us:
"water is unphysiological and beer is expensive,
so we use saline."  He must have been a good 
lecturer because I can still recall that 
apophthegm 40 years on when afar and asunder!

John Kiernan
London, Ontario.
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> Hi All,
> I am new to fluorescence immunohistochemistry and I was wondering if
> anyone can let me know the simple steps involved in performing this
> technique. I know the dilutions required for the primary and secondary
> antibodies, I just need to known what solutions are used for washes and
> the steps in between!
> Thanks in advance,