[Histonet] MMA sections coming off!

[Tracey Couse]

Patricia,

Try manually coating plain glass slides with poly-l-lysine, 
chrome/gelatin, or glycerin/gelatin.  We use all of these for our thin 
plastic sections.  I also suggest setioning a micron or two thinner (cut 
at 3 or 4um).  A thinner section will reduce the surface area to volume 
ratio and may promote better section adherance.  You may need to dry the 
sldies using a slide press and dry longer or with higher temperatures. 
Also, try searching the Histonet archives for alternative slide coating 
suggestions.  Good luck!

-- 
Tracey Couse
Laboratory Coordinator
Georgia Tech/Emory Center for the
      Engineering of Living Tissues
Georgia Institute of Technology
IBB, Room 1123
Office: 404.385.2611
Lab: 404-385-6735
Fax: 404.894.229


Date: Mon, 19 Dec 2005 14:21:07 -0600
From: "Patricia F Lott" <plott <@t> uab.edu>
Subject: [Histonet] MMA sections coming off!
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<C4BBA8D04B6FAD47B10C5AFF4BBB93FD026F32EE <@t> UABEXMB2.ad.uab.edu>
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Help!  We are losing our thin plastic sections!  We have cut PMMA
plastic sections at 5 microns and put them on "+" slides, and the
sections fall off either during de-plasticizing or during staining!  We
have tried:  Haupt's adhesive, longer drying times, "+" slides, plus
slides and Haupt's together, etc....no luck!  Any suggestions would be
welcome!
1