[Histonet] MMA sections coming off!
tracey.couse <@t> ibb.gatech.edu
Tue Dec 20 14:15:42 CST 2005
Try manually coating plain glass slides with poly-l-lysine,
chrome/gelatin, or glycerin/gelatin. We use all of these for our thin
plastic sections. I also suggest setioning a micron or two thinner (cut
at 3 or 4um). A thinner section will reduce the surface area to volume
ratio and may promote better section adherance. You may need to dry the
sldies using a slide press and dry longer or with higher temperatures.
Also, try searching the Histonet archives for alternative slide coating
suggestions. Good luck!
Georgia Tech/Emory Center for the
Engineering of Living Tissues
Georgia Institute of Technology
IBB, Room 1123
Date: Mon, 19 Dec 2005 14:21:07 -0600
From: "Patricia F Lott" <plott <@t> uab.edu>
Subject: [Histonet] MMA sections coming off!
To: <Histonet <@t> lists.utsouthwestern.edu>
<C4BBA8D04B6FAD47B10C5AFF4BBB93FD026F32EE <@t> UABEXMB2.ad.uab.edu>
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Help! We are losing our thin plastic sections! We have cut PMMA
plastic sections at 5 microns and put them on "+" slides, and the
sections fall off either during de-plasticizing or during staining! We
have tried: Haupt's adhesive, longer drying times, "+" slides, plus
slides and Haupt's together, etc....no luck! Any suggestions would be
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