[Histonet] preservation of unstained 20 um sections on slides for later DNA research

[Helen Fedor]

We have had pretty good success with drying them overnight, vertically, then  putting them face to face, wrapping them tightly in parafilm and then putting them into a ziploc and expelling the air.
And then store at -20.
We have only done this on 4 to 5 micron sections, and have them stored for up to 3 years and they retain their antigenicity fairly well.
Helen

Helen L. Fedor B.S.
Johns Hopkins University
Pathology Department
600 N Wolfe St
Marburg Room 406
Baltimore MD 21287
email: hfedor <@t> jhmi.edu
Phone: 410 614-1660
Pager: 410 283-3419

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