[Histonet] method of using agar for an embedding tool

[Edmondson David (RBV) NHS Christie Tr]

There was a report in the Journal of Med Lab Tech, in the 70s that described use of agar to embedd small bits, 
Cut the end off plastic syringes to reveal the whole bore. 
Clip or otherwise mount the syringe upright and pull back to leave say 3mm above the plunger.
2% agar, meleted in a microwave for 20 secs. 
Pipette in agar
Orient tissue
Allow to cool
syringe depressed and block come out 
Ready to go in a cassette
Bob's your uncle

If you would like the reference then RSVP
David
Christie Hospital
Manchester UK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Vickroy,
Jim
Sent: 15 December 2005 20:50
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] method of using agar for an embedding tool


 

Has anyone used agar at the embedding stations to help orient small
specimens?  I am told by my new pathology assistant that this would
prevent the embedders from having to reorient them at the embedding
center?  If you have could someone send me a procedure?  Please
disregard the spelling errors in the last email.  Obviously spell check
should have been used.

 

James R. Vickroy

Supervisor - MMC Surgical Pathology

217-788-4046

 



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