[Histonet] RE: DAP vs Hoescht

[Andrea T. Hooper]

Dear Sarah,

I must respectfully disagree.

I use Hoechst 33342 diluted to 0.1ug/ml for 10 minutes on a daily 
basis with various aqueous mountants including Pro-Long Gold (I agree 
with Gayle that it is a good one), Slow Fade, Vectashield, 
Fluormount-G, Gelmount etc etc. Works like a charm, never a problem, 
gorgeous counterstain

Sincerely,
Andrea


At 1:30 PM -0500 12/15/05, Pixley, Sarah (pixleysk) wrote:
>Dear Caroline:
>
>We have found that both are very easy to use, but the problem with
>Hoechst is that it CANNOT be used with an aqueous mounting medium. Both
>can be purchased as the relatively cheap solutions  (i.e., DAPI:  Mol.
>Probes D-1306; Hoechst 33258: Sigma catalog # B2883). Both can be
>diluted in an aqueous buffer (PB or PBS) and then you incubate your
>sections for 5 mins, then rinse and coverslip. But DAPI can be used with
>any aqueous mounting medium, like Gelvatol or Fluoromount, etc., while
>with Hoechst, you have to dehydrate, go to xylene or its equivalent and
>mount with a standard mounting medium like Permount or Depex.
>Hope this helps.
>Sarah Pixley
>
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