[Histonet] adjusting pH for formalin
Rene J Buesa
rjbuesa <@t> yahoo.com
Thu Dec 15 14:18:51 CST 2005
Any strong base (like sodium hydroxide) could be used. The reaction will increase the
pH value and sodium will end as a sodium salt with no effects on fixation.
Another solution would be to use phosphate salts to reconstitute the "classical" 10%
NBF (neutral buffered formalin).
René J.
Vaughn Fierke <Vfierke <@t> SNBLUSA.com> wrote:
Anyone with an idea to bring up the pH of 10% formalin to 7.0. Our
recycler tests 5 gallon batches at 6.2 and the paths would like
everything at 7.0. One source says add 1% sodium phosphate dibasic;
others say use SP dibasic saturated solution. Another source says that
using 1% sodium hydroxide is ok and won't alter fixation. Any consistent
suggestions?
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