[Histonet] F4/80, IL-12 flourescent

Thomas Pier tp2 <@t> medicine.wisc.edu
Thu Dec 15 10:33:10 CST 2005


Hello,
I hope that everyone is doing well.  My current project requires me to
stain frozen mouse tumors for F4/80 and IL-12.  I was wondering if
anyone out there has a good protocol for flourescent staining of either
of these antibodies.  The antibodies that were provided for me are for
flow or westerns.  They have not given me good results (not that that's
a shocker).  Buying IHC specific antibodies is probably going to be the
next step, so if anybody knows of some good ones please let me know. 
Thanks alot.  Happy Holidays.

Tom Pier



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