[Histonet] Double fluorescence labelling of paraffin sections

[Gayle Callis]

Did you try the Molecular Probes autofluorescence blocking 
reagent?  Image-iT FX signal enhancer?

At 07:07 AM 12/15/2005, you wrote:
>Hello,
>
>does anyone have any suggestions how to eliminate background fluorescence 
>of paraffin slices? I tried the autofluorescence eliminator, but that 
>wasnt enough.
>Maybe the deparaffinization is not well enough with Rotihistol?
>Thanks,
>Nicole Klapka
>
>--
>Nicole Klapka PhD
>Labor für Molekulare Neurobiologie
>TVA, Geb. 22.22
>Heinrich-Heine Universität Düsseldorf
>Universitätsstr. 1
>40225 Düsseldorf
>Tel: ++49 211 81 14437
>
>
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)