[Histonet] Double fluorescence labelling of paraffin sections

Gayle Callis gcallis <@t> montana.edu
Thu Dec 15 10:17:33 CST 2005

Did you try the Molecular Probes autofluorescence blocking 
reagent?  Image-iT FX signal enhancer?

At 07:07 AM 12/15/2005, you wrote:
>does anyone have any suggestions how to eliminate background fluorescence 
>of paraffin slices? I tried the autofluorescence eliminator, but that 
>wasnt enough.
>Maybe the deparaffinization is not well enough with Rotihistol?
>Nicole Klapka
>Nicole Klapka PhD
>Labor für Molekulare Neurobiologie
>TVA, Geb. 22.22
>Heinrich-Heine Universität Düsseldorf
>Universitätsstr. 1
>40225 Düsseldorf
>Tel: ++49 211 81 14437
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

More information about the Histonet mailing list