[Histonet] Myelin in spinal cord

[John A. Kiernan]

Ordinary myelin stains work well on paraffin
sections of formaldehyde-fixed tissue. Are you
doing some additional method that works only with
cryostat sections?

Another factor may be age. If the fishes are very
young, they may not yet have  myelin sheaths in
their central nervous systems.
-- 
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
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"germckeon <@t> excite.com" wrote:
> 
> Hello,
> 
> I have a problem and am in need of some advice.
> 
> I am working on spinal cord of zebrafish and will be staining for
> 
> myelin in fine detail. I have been strongly advised not to use paraffin embedding and so am currently doing gelatin embedded sectioning on a cryostat. Unfortunately this has led to tissue loss due to poor adherence to the slide. I tried superfrost plus and superfrost gold slides with no change. gelatin coating the slides
> 
> greatly improved this but staining clarity and specifity is very poor. I have tried varying times and even stains (Kultschitsky's verses Weil's) but no improvement.
> 
> Does anyone have any suggestions on how to improve this or what other other techniques (including possibly other stains) I could try?
> 
> Thanking you,
> 
> Gerald McKeon
>