[Histonet] Re: Myelin in spinal cord

[Gayle Callis]

I'm curious as to why paraffin was not advised?  Reasons?

We had terrible luck with frozen sections and prefer to do paraffin 
sections for myelin - one can either do a Kluver Barrera luxol fast blue or 
you can do John Kiernans solochrome cyanin (gives you black myelin instead 
of blue).  There are many new myelin staining technics out there, some are 
fluorescent and a just published method in Dec issue of J Histochemistry 
Cytochemistry.   Check out this journal for other myelin staining methods 
too.   Molecular Probes has another fluorescent myelin staining kit - go to 
their website to check on what they offer.


Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)