[Histonet] Cuting speed and sem-thin plastic sectioning
Lawrence D Lanberg/O/VCU
lanbergld <@t> vcu.edu
Tue Dec 13 19:18:21 CST 2005
I am a beginning microtomist - most part - in a Biochemistry laborato retinas using plastic (Spurr's Formula).&nbs MT-1000 ultramicrotome. My sectioning, staining and developed very well in the 4 months I have done this. I have little thing, though, that seems to be impeding progress: Speed of
Currently, the only way I get quality sec mm/sec. Which is almost the slowest setting on t lucky, I may get three blocks done per hour; 20 section slides per block. This is slow output, from what I am told.
I have experimented cutting these on a speed as high This greatly increases my output, obviously, but after staining-destaining the sections appear corrugated. But they are
I am un cordurouy-like plastic (hard formulation self-made glass knives, can anybody possibly cut on the higher speeds, while mai section?
Student Worker IST
Virginia Commonwealth University
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