[Histonet] Another Histonet Archive CD31 for murine FFPE tissue
gcallis <@t> montana.edu
Tue Dec 13 11:34:11 CST 2005
It seems most people use enzyme digestion with FFPE CD31 on mouse, rather
than an antigen retrieval i.e. with buffers/HIER.
I just completed a project usind CD31 on FFPE mouse tissue with good results.
The supplier of the antibody is Research Diagnostics (973-584-7093;
www.researchd.com). I used rat anti-mouse CD31, monoclonal; Catalog#
RDI-mCD31abrt. They also suggested zinc fixation, but it worked on formalin
fixation. I used DAKO's Prot K to open up the sights.
Pathology Core Facility
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
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