[Histonet] RE: Histonet Digest, Vol 25, Issue 12

Tracey Dunn Tracey <@t> labcareer.com
Fri Dec 9 12:24:48 CST 2005


Can't you call me on a break or something ? 


Tracey

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, December 09, 2005 1:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 25, Issue 12

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Today's Topics:

   1. microwriters (Webb, Dorothy L)
   2. RE: microwriters (Weems, Joyce)
   3. formalin waste disposal (JPaulB42 <@t> aol.com)
   4. re: BrdU on cultured cells (Kevin Conway)
   5. Re: Mast cells in spinal cord (John Kiernan)
   6. cap ihc info (Roxanne Soto)
   7. RE: cap ihc info (patsy ruegg)
   8. RE: formalin waste disposal (patsy ruegg)
   9. RE: formalin waste disposal (Weems, Joyce)
  10. RE: formalin waste disposal (Weems, Joyce)
  11. RE: cap ihc info (Drew Sally A.)
  12. Re: cap ihc info (Dana Settembre)
  13. RE: cap ihc info (Dawson, Glen)
  14. RE: cap ihc info (Favara, Cynthia (NIH/NIAID) [E])


----------------------------------------------------------------------

Message: 1
Date: Thu, 08 Dec 2005 13:19:50 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] microwriters
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<0E394B648E5284478A6CCB78E5AFDA2718C4FE <@t> hpes1.HealthPartners.int>
Content-Type: text/plain;	charset="us-ascii"

Does anyone have a system in use that labels cassettes and slides and is
hooked into your computer system, I believe they are called
microwriters?  They have available to the system a bar code reader.  We
are looking to go that route in 2006, but wondering of some feedback
from users!!

Also, how long do you use your recycled xylene?  Do you have outdates or
expiration dates on the recycled xylene?  An inspector for JACHO asked
the question, and we were not able to give a good answer!
________________________________________
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If you have received this e-mail in error, please immediately notify the
HealthPartners Support Center by telephone at (952) 967-6600. You will
be reimbursed for reasonable costs incurred in notifying us.


------------------------------

Message: 2
Date: Thu, 8 Dec 2005 14:31:06 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] microwriters
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<83AACDB0810528418AA106F9AE9B7F7E0130524A <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;  charset="utf-8"

We have Triple G LIS - and just yesterday completed interface with
Sakura cassette and slide printers. It is amazing - bar code and all.
Barcoding is not active yet, but will be with next version of our LIS. 

As far as recycled xylene - I will that to a better expert. To my
knowledge it never expires! 

Joyce


Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Webb,
Dorothy L
Sent: Thursday, December 08, 2005 2:20 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] microwriters

Does anyone have a system in use that labels cassettes and slides and is
hooked into your computer system, I believe they are called
microwriters?  They have available to the system a bar code reader.  We
are looking to go that route in 2006, but wondering of some feedback
from users!!

Also, how long do you use your recycled xylene?  Do you have outdates or
expiration dates on the recycled xylene?  An inspector for JACHO asked
the question, and we were not able to give a good answer!
________________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please
be advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is
strictly prohibited.

If you have received this e-mail in error, please immediately notify the
HealthPartners Support Center by telephone at (952) 967-6600. You will
be reimbursed for reasonable costs incurred in notifying us.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may
be privileged and is confidential information intended for the use of
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received this communication in error, please notify us immediately by
replying to the message and deleting it from your computer. Thank you.
Saint Joseph's Health System, Inc.

------------------------------

Message: 3
Date: Thu, 8 Dec 2005 15:52:25 EST
From: JPaulB42 <@t> aol.com
Subject: [Histonet] formalin waste disposal
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <238.338efba.30c9f709 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Dear Histonetters:
 
     I am looking for information on any company that  picks up tissue
specimens with formalin still on the specimen for disposal. We  were
recently reclassified by the EPA and need to make immediate changes in
formalin disposal, alcohol disposal and xylene disposal. Any help would
be  appreciated.
 
Jim Bradford
Orlando Regional Medical Center
Orlando, FL


------------------------------

Message: 4
Date: Thu, 08 Dec 2005 16:13:30 -0500
From: "Kevin Conway" <kconway <@t> cmcc.ca>
Subject: [Histonet] re: BrdU on cultured cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s3985bc7.060 <@t> mail.cmcc.ca>
Content-Type: text/plain; charset=US-ASCII

Hi Steve,

In addition to permeabilizing (we used .15% Triton X-100, no reason the
saponin wouldn't also work), I added a denaturation step (x 10 min in ?2
N HCl (aq)- I will double-check the [HCl] and let you know for sure if
you don't have a protocol. Just helps to keep the histones away :)

Also, I'm not sure what antibody you're using, but we always had great
success with the G3G4 Mab from DSHB.
Good luck,

Kevin
--------------------------------------

Date: Wed, 7 Dec 2005 12:31:10 -0800 (PST)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] Brdu staining fixed cultured cells.


Good afternoon everyone,
   
  I'm working witha scientist who is attempting to stain previously
fixed cultured cells in "wells" and cover slips with anti-Brdu.  He has
fixed the cells 1st in methanol, air dryed, then in 10%formalin/PBS and
again air dryed.  Our anti-BrdU/DNAase works great on tissue but not on
the fixed/air dryed cultured cells.  We tried a 30 minute 0.1% Saponin
step to help permeablize the cells but w/o success.  Any ideas.
   
  Steve

			
---------------------------------


Kevin Conway, PhD
Assistant Professor
Department of Anatomy
Canadian Memorial Chiropractic College
416-482-2340 x.258
kconway <@t> cmcc.ca



------------------------------

Message: 5
Date: Thu, 08 Dec 2005 23:50:48 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: [Histonet] Re: Mast cells in spinal cord
To: clcun <@t> itsa.ucsf.edu
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <43990D28.2BACCB0A <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1

Dear Dr Cun,

You need to do some more homework! You probably found my name because of
two publications:
Campbell, D. J. and Kiernan, J. A. 1966. Mast cells in the central
nervous system. Nature 210, 756-757. 
Kiernan, J. A. 1976. A comparative survey of the mast cells of the
mammalian brain. Journal of Anatomy 121, 303-311.

These relate to mast cells in the normal CNS (which occur in only a few
species, if you exclude the ones in connective tissue around blood
vessels). In central nervous tissue, in contact with neurons and glial
cells, mast cells occur only in the habenular nuclei and nearby
dorsomedial thalamus, in some Insectivora and "lower" primates
(tree-shrew, loris). 
In the 1990s Zhuang, Silver and others published a series of papers
about mast cells in the medial habenular nucleus of birds
- the place where they are most abundant in insectivores and prosimians.


To the best of my knowledge, mast cells do not occur in the normal
spinal cord of any vertebrate animal. Is your research with the normal
or diseased spinal cord? 

Mast cells are abundant in the dura mater. All research with mast cells
begins with a reading of Hans Selye's book "The Mast Cells"
(1963). Have you consulted this book? It contains the answers to most of
your questions. The fixation and staining problems had all been nailed
down by 1963. For most species you are wasting your time trying to find
mast cells in frozen/cryostat sections.
The tryptase and chymase activities do not exist in all species.
Rodents differ from humans and dogs. 

Discharged mast cell granules
 may hang around and be stainable as extracellular dots (rat), or they
may quickly dissolve and contribute to anaphlaxis (dog, guinea-pig, some
people). All this was known 40 years ago. 

John Kiernan
Anatomy & Cell Biology
London, Canada.
______________________________________
clcun <@t> itsa.ucsf.edu wrote:
> 
> Dear Dr. Kiernan,
> 
> I am trying to study mast cells in the CNS of mice and your name has 
> popped up on several occasions.  I have a few questions regarding 
> proper prepping/ staining of mast cells and would greatly appreciate 
> your expertise on this matter.
> 
> I would like to locate mast cells in the spinal cord using several
stains:
> toludine blue, chloroacetate esterase, cKIT (ACK45) 
> immunohistochemistry, tryptase and chymase enzyme histochemistry.
> 
> 1. What fixative is suitable for these stains?  We normally perfuse 
> our animals with 4% paraformaldehyde and cryoprotect our tissues in 
> 30% sucrose, but we've heard that this may not work for some of these 
> staining protocols (especially, cKIT immuno and tryptase/chymase 
> enzyme histo, which some have recommended using frozen tissues).  We 
> would like to minimize usage of different fixation methods, so it 
> would be great if we could get away with using paraformaldehyde, but 
> only if it will give us good preservation of mast cells.
> 
> 2. What is the best way to embed our tissues?  After fixation, we 
> usually cryoprotect with sucrose and block our tissues in OCT, but 
> some people have suggested paraffin embedded tissues.  Unfortunately, 
> our lab is not equipped to do paraffin embedding.  Would we be able to

> get good sections with our cryoprotected OCT blocked tissues?
> 
> 3.  Which orientation would be best to see the distribution of mast 
> cells in the spinal cord, longitudinal or cross-section?
> 
> 4. Of the above stains that I've mentioned, which one do you think is 
> best, in terms of specificity for mast cells, ease, etc.
> 
> Any comments and suggestions you can give would be greatly
appreciated.
> Thank you in advance for your time.
> 
> Sincerely,
> 
> Christine Cun
> Department of Neurosurgery
> University of California, San Francisco




------------------------------

Message: 6
Date: Fri, 09 Dec 2005 10:18:39 -0500
From: "Roxanne Soto" <godsgirlnow <@t> msn.com>
Subject: [Histonet] cap ihc info
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY106-F25F2F0B5828C3E963D619ABC450 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"


       ______________________________________________________________

     >I  have  a  question  about  a particular IHC that I do on a daily
     basis. I do a PIN-4 cocktail
     >stain  on  prostate  cores. This cocktail contains p63, CK903, and
     P504S. My question is, do I
     >need to perform a negative control?
     >Right  now  we are not CAP certified, but we are looking to become
     certified. CLIA only
     >requires one negative per case, instead of one negative per block.
     We deal with very tiny
     >cores  and  I  am  currently following the CLIA guidelines in this
     matter. However, we are
     >looking  to  automate  and  work  on  CAP  accreditation  and this
     negative factor would greatly
     >increase  our volume and therefor would mean that we would need to
     get a stainer to
     >accommodate the volume.
     >I  know that whenever I do a CK903, I do not do a negative because
     the tissue has built in
     >controls. Is this now null and void because of the racemase?
     >Please advice.
     >Thank you
     >Roxanne Soto HT(ASCP)
     >Tampa


------------------------------

Message: 7
Date: Fri, 9 Dec 2005 09:30:07 -0700
From: "patsy ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] cap ihc info
To: "'Roxanne Soto'" <godsgirlnow <@t> msn.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200512091630.jB9GU6ch068463 <@t> pro12.abac.com>
Content-Type: text/plain;	charset="US-ASCII"

This is an ongoing issue in IHC, when dealing with limited tissue how
can we afford to use up sections for negative controls on each block.  I
haven't been updated on this for awhile, just wondering what is
happening in the real world, are you including a negative (isotype
matched reagent in place of the primary antibody) for each block even
when it is a small biopsy specimen.
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roxanne
Soto
Sent: Friday, December 09, 2005 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cap ihc info


       ______________________________________________________________

     >I  have  a  question  about  a particular IHC that I do on a daily
     basis. I do a PIN-4 cocktail
     >stain  on  prostate  cores. This cocktail contains p63, CK903, and
     P504S. My question is, do I
     >need to perform a negative control?
     >Right  now  we are not CAP certified, but we are looking to become
     certified. CLIA only
     >requires one negative per case, instead of one negative per block.
     We deal with very tiny
     >cores  and  I  am  currently following the CLIA guidelines in this
     matter. However, we are
     >looking  to  automate  and  work  on  CAP  accreditation  and this
     negative factor would greatly
     >increase  our volume and therefor would mean that we would need to
     get a stainer to
     >accommodate the volume.
     >I  know that whenever I do a CK903, I do not do a negative because
     the tissue has built in
     >controls. Is this now null and void because of the racemase?
     >Please advice.
     >Thank you
     >Roxanne Soto HT(ASCP)
     >Tampa
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 8
Date: Fri, 9 Dec 2005 09:32:44 -0700
From: "patsy ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] formalin waste disposal
To: <JPaulB42 <@t> aol.com>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200512091632.jB9GWh7t070031 <@t> pro12.abac.com>
Content-Type: text/plain;	charset="US-ASCII"

We always had to pour the formalin into separate disposal containers,
then
dispose of the tissue as biohazard.  The tissue was picked up by someone
and
the chemical waste by another, they did not combine services.  It has
been a
while since I looked into this, perhaps someone has met that service
need by
now.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
JPaulB42 <@t> aol.com
Sent: Thursday, December 08, 2005 1:52 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] formalin waste disposal

Dear Histonetters:
 
     I am looking for information on any company that  picks up tissue 
specimens with formalin still on the specimen for disposal. We  were
recently 
reclassified by the EPA and need to make immediate changes in  formalin
disposal, 
alcohol disposal and xylene disposal. Any help would be  appreciated.
 
Jim Bradford
Orlando Regional Medical Center
Orlando, FL
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 9
Date: Fri, 9 Dec 2005 11:47:25 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] formalin waste disposal
To: "patsy ruegg" <pruegg <@t> ihctech.net>, <JPaulB42 <@t> aol.com>,
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<83AACDB0810528418AA106F9AE9B7F7E01305268 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;  charset="utf-8"

We have biohazard disposal on site and dispose of small containers in
biohazard bags to which Tissue-Tek neutralizer has been added. We pour
formalin off large specimens and neutralize with Tissue-Tek neutralizer.
This is approved by CA EPA so we think it has to be safe.  

Happy Friday! j:>)


Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of patsy
ruegg
Sent: Friday, December 09, 2005 11:33 AM
To: JPaulB42 <@t> aol.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] formalin waste disposal

We always had to pour the formalin into separate disposal containers,
then
dispose of the tissue as biohazard.  The tissue was picked up by someone
and
the chemical waste by another, they did not combine services.  It has
been a
while since I looked into this, perhaps someone has met that service
need by
now.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
JPaulB42 <@t> aol.com
Sent: Thursday, December 08, 2005 1:52 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] formalin waste disposal

Dear Histonetters:

     I am looking for information on any company that  picks up tissue
specimens with formalin still on the specimen for disposal. We  were
recently
reclassified by the EPA and need to make immediate changes in  formalin
disposal,
alcohol disposal and xylene disposal. Any help would be  appreciated.

Jim Bradford
Orlando Regional Medical Center
Orlando, FL
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may
be privileged and is confidential information intended for the use of
the addressee listed above. If you are neither the intended recipient
nor the employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any disclosure,
copying, distribution or the taking of any action in reliance on the
contents of this information is strictly prohibited. If you have
received this communication in error, please notify us immediately by
replying to the message and deleting it from your computer. Thank you.
Saint Joseph's Health System, Inc.

------------------------------

Message: 10
Date: Fri, 9 Dec 2005 11:58:38 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] formalin waste disposal
To: "Weems, Joyce" <JWEEMS <@t> sjha.org>, "patsy ruegg"
	<pruegg <@t> ihctech.net>,	<JPaulB42 <@t> aol.com>,
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<83AACDB0810528418AA106F9AE9B7F7E0130526A <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;  charset="utf-8"

And I should have said - dispose of neutralized formalin down the drain.



Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Weems,
Joyce
Sent: Friday, December 09, 2005 11:47 AM
To: patsy ruegg; JPaulB42 <@t> aol.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] formalin waste disposal

We have biohazard disposal on site and dispose of small containers in
biohazard bags to which Tissue-Tek neutralizer has been added. We pour
formalin off large specimens and neutralize with Tissue-Tek neutralizer.
This is approved by CA EPA so we think it has to be safe. 

Happy Friday! j:>)


Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of patsy
ruegg
Sent: Friday, December 09, 2005 11:33 AM
To: JPaulB42 <@t> aol.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] formalin waste disposal

We always had to pour the formalin into separate disposal containers,
then
dispose of the tissue as biohazard.  The tissue was picked up by someone
and
the chemical waste by another, they did not combine services.  It has
been a
while since I looked into this, perhaps someone has met that service
need by
now.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
JPaulB42 <@t> aol.com
Sent: Thursday, December 08, 2005 1:52 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] formalin waste disposal

Dear Histonetters:

     I am looking for information on any company that  picks up tissue
specimens with formalin still on the specimen for disposal. We  were
recently
reclassified by the EPA and need to make immediate changes in  formalin
disposal,
alcohol disposal and xylene disposal. Any help would be  appreciated.

Jim Bradford
Orlando Regional Medical Center
Orlando, FL
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may
be privileged and is confidential information intended for the use of
the addressee listed above. If you are neither the intended recipient
nor the employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any disclosure,
copying, distribution or the taking of any action in reliance on the
contents of this information is strictly prohibited. If you have
received this communication in error, please notify us immediately by
replying to the message and deleting it from your computer. Thank you.
Saint Joseph's Health System, Inc.


Confidentiality Notice ** The information contained in this message may
be privileged and is confidential information intended for the use of
the addressee listed above. If you are neither the intended recipient
nor the employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any disclosure,
copying, distribution or the taking of any action in reliance on the
contents of this information is strictly prohibited. If you have
received this communication in error, please notify us immediately by
replying to the message and deleting it from your computer. Thank you.
Saint Joseph's Health System, Inc.

------------------------------

Message: 11
Date: Fri, 9 Dec 2005 11:03:57 -0600
From: "Drew Sally A." <sa.drew <@t> hosp.wisc.edu>
Subject: RE: [Histonet] cap ihc info
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
	
<D6B654003615874B873E15BA680E2D2213C9C1B2 <@t> uwhis-xchng1.hosp.wisc.edu>
Content-Type: text/plain;	charset="us-ascii"

We have recently changed how we deal with the negative control
situation.
We run one negative control slide per specimen block, using our most
aggressive pretreatment
protocol, per panel of antibodies.  We also document that within our
positive control tissue 
there are negative tissue elements.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of patsy
ruegg
Sent: Friday, December 09, 2005 10:30 AM
To: 'Roxanne Soto'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] cap ihc info


This is an ongoing issue in IHC, when dealing with limited tissue how
can we afford to use up sections for negative controls on each block.  I
haven't been updated on this for awhile, just wondering what is
happening in the real world, are you including a negative (isotype
matched reagent in place of the primary antibody) for each block even
when it is a small biopsy specimen. Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roxanne
Soto
Sent: Friday, December 09, 2005 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cap ihc info


       ______________________________________________________________

     >I  have  a  question  about  a particular IHC that I do on a daily
     basis. I do a PIN-4 cocktail
     >stain  on  prostate  cores. This cocktail contains p63, CK903, and
     P504S. My question is, do I
     >need to perform a negative control?
     >Right  now  we are not CAP certified, but we are looking to become
     certified. CLIA only
     >requires one negative per case, instead of one negative per block.
     We deal with very tiny
     >cores  and  I  am  currently following the CLIA guidelines in this
     matter. However, we are
     >looking  to  automate  and  work  on  CAP  accreditation  and this
     negative factor would greatly
     >increase  our volume and therefor would mean that we would need to
     get a stainer to
     >accommodate the volume.
     >I  know that whenever I do a CK903, I do not do a negative because
     the tissue has built in
     >controls. Is this now null and void because of the racemase?
     >Please advice.
     >Thank you
     >Roxanne Soto HT(ASCP)
     >Tampa
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------------------------------

Message: 12
Date: Fri, 09 Dec 2005 10:43:05 -0500
From: Dana Settembre <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] cap ihc info
To: histonet <@t> lists.utsouthwestern.edu, godsgirlnow <@t> msn.com
Message-ID: <s3995fd7.086 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII

Roxanne,
Most of your wondering is correct, such as using a negative control per
block.  Your volume will increase.  In reference to "built in negative
control" you should have that specifically mentioned in your procedure
manual. The very best thing to do is to ask your specific question(s) to
CAP directly. They have a e-mail address that I have use several times
and they respond promptly and precisely.  Here's the address:
accred <@t> cap.org 

Good Luck,
Dana Settembre
University Hospital - UMDNJ
Newark, New Jersey

>>> Roxanne Soto <godsgirlnow <@t> msn.com> 12/9/2005 10:18:39 AM >>>

       ______________________________________________________________

     >I  have  a  question  about  a particular IHC that I do on a
daily
     basis. I do a PIN-4 cocktail
     >stain  on  prostate  cores. This cocktail contains p63, CK903,
and
     P504S. My question is, do I
     >need to perform a negative control?
     >Right  now  we are not CAP certified, but we are looking to
become
     certified. CLIA only
     >requires one negative per case, instead of one negative per
block.
     We deal with very tiny
     >cores  and  I  am  currently following the CLIA guidelines in
this
     matter. However, we are
     >looking  to  automate  and  work  on  CAP  accreditation  and
this
     negative factor would greatly
     >increase  our volume and therefor would mean that we would need
to
     get a stainer to
     >accommodate the volume.
     >I  know that whenever I do a CK903, I do not do a negative
because
     the tissue has built in
     >controls. Is this now null and void because of the racemase?
     >Please advice.
     >Thank you
     >Roxanne Soto HT(ASCP)
     >Tampa
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 13
Date: Fri, 9 Dec 2005 11:44:39 -0600 
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] cap ihc info
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <D2401DE71F59D71184BC00D0B7479B91013645D3 <@t> MILW_MAIL1>
Content-Type: text/plain;	charset="iso-8859-1"

All,

Just an Observation...Has anyone else ever wondered why we cannot charge
for
IHC's on multiple blocks that are considered the "same" sample: eg. A1,
A2,
A3, A4, A5 on a case where 3 IHC's are ordered on each block, only 3 are
billable although we are really performing 15.  Yet, when it comes to
negative controls, one must be run on each of the blocks because they
are
seperate and distinct.

The end result of this madness is that, if you count the negative
control
run on each of the blocks, although 20 IHC's are run for the above case,
only 3 are billable.  I find it strange that when it comes to being able
to
bill for a case like this, the blocks are too much
the "same" to bill for all technical work done, but,when it comes to
running
negatives (& lets face it, negatives are largely procedural and not
closely
monitored by many pathologists), it is vital that one be run on each
block
because they are not the same, although it is all just one, big, happy
specimen.  The IHC lab is lucky enough to take it in the shorts on cases
like this.

Ain't Life Grand,

Glen Dawson
Milwaukee, WI



------------------------------

Message: 14
Date: Fri, 9 Dec 2005 12:49:47 -0500
From: "Favara, Cynthia \(NIH/NIAID\) [E]" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] cap ihc info
To: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>,	"Histonet"
	<histonet <@t> pathology.swmed.edu>
Message-ID:
	<A985B7D45D355D4EB363288E717394375B80CD <@t> NIHCESMLBX5.nih.gov>
Content-Type: text/plain;	charset="us-ascii"

Because like so many other things the people that made the rule have no
idea what is going on!

Have a good weekend!

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Dawson, Glen [mailto:GDawson <@t> dynacaremilwaukee.com] 
Sent: Friday, December 09, 2005 10:45 AM
To: Histonet
Subject: RE: [Histonet] cap ihc info

All,

Just an Observation...Has anyone else ever wondered why we cannot charge
for
IHC's on multiple blocks that are considered the "same" sample: eg. A1,
A2,
A3, A4, A5 on a case where 3 IHC's are ordered on each block, only 3 are
billable although we are really performing 15.  Yet, when it comes to
negative controls, one must be run on each of the blocks because they
are
seperate and distinct.

The end result of this madness is that, if you count the negative
control
run on each of the blocks, although 20 IHC's are run for the above case,
only 3 are billable.  I find it strange that when it comes to being able
to
bill for a case like this, the blocks are too much
the "same" to bill for all technical work done, but,when it comes to
running
negatives (& lets face it, negatives are largely procedural and not
closely
monitored by many pathologists), it is vital that one be run on each
block
because they are not the same, although it is all just one, big, happy
specimen.  The IHC lab is lucky enough to take it in the shorts on cases
like this.

Ain't Life Grand,

Glen Dawson
Milwaukee, WI

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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

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End of Histonet Digest, Vol 25, Issue 12
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