[Histonet] coverslipping bubbles

[Favara, Cynthia (NIH/NIAID) [E]]

I was wondering if anyone has tried a wetting agent in the distilled
water prior to coverslipping with aqueous mounting media? If so what did
you use and at what concentration. I just thought it might help with my
constant bubble problem on immunos.

 

Thanks,

c

 

Cynthia Favara 
NIAID/NIH/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
406-363-9317 

Disclaimer: 
The information in this e-mail and any of its attachments is
confidential and may contain sensitive information. It should not be
used by anyone who is not the original intended recipient. If you have
received this e-mail in error please inform the sender and delete it
from your mailbox or any other storage devices. National Institute of
Allergy and Infectious Diseases shall not accept liability for any
statements made that are sender's own and not expressly made on behalf
of the NIAID by one of its representatives