[Histonet] RE: Histonet Digest, Vol 25, Issue 8
Rice, Michael
Michael.Rice <@t> holy-cross.com
Tue Dec 6 13:49:34 CST 2005
Hi Glen, We are using USLABS and getting results back in 4-5 days. We also use them for flow and have results back in 24-48 hours. They are great to work with and no, I do not having any financial interest in them
Mike rice
Holy cross hospital
Ft lauderdale
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 06, 2005 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 25, Issue 8
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Today's Topics:
1. Looking for Dr. Valerie Ray (pathrm35 <@t> adelphia.net)
2. RE: active Caspase-3 antibody and TUNEL kit (Melissa Gonzalez)
3. re:MSH-2 and MLH-1 (Emma JONES)
4. specific Ig detection (JEFF TATUM ZELIADT)
5. Re: specific Ig detection (Gayle Callis)
6. Anti-DIG peroxidase problem (Mikael Niku)
7. RE: Histonet Digest, Vol 25, Issue 5 (Gus Mondragon)
8. Bone EM Question (Sheffield, Tiffany L)
9. FISH for HER2 testing (Dawson, Glen)
10. Tropheryma whippleii (ole)
11. Re: FISH for HER2 testing (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Mon, 5 Dec 2005 13:32:14 -0500
From: <pathrm35 <@t> adelphia.net>
Subject: [Histonet] Looking for Dr. Valerie Ray
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5120670.1133807534399.JavaMail.root <@t> web6.mail.adelphia.net>
Content-Type: text/plain; charset=utf-8
I am trying to contact Dr. Valerie Anne Yantsos Ray from the Tampa Bay, Florida area. If anyone knows her, please forward this email to her as I would like to speak with her.
Thanks in advance.
Ron Martin
561-721-2400
------------------------------
Message: 2
Date: Mon, 5 Dec 2005 10:46:33 -0800
From: "Melissa Gonzalez" <Melissa.Gonzalez <@t> cellgenesys.com>
Subject: [Histonet] RE: active Caspase-3 antibody and TUNEL kit
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: tenny jin <tennyjin <@t> gmail.com>
Message-ID:
<A6B0506C2B000E45A7556B9A93C4554707792C <@t> hqsvr01mail.cgi.com>
Content-Type: text/plain; charset="us-ascii"
Tenny,
Try the TUNEL kit from Chemicon, and active Caspase3 from R&D Systems
(not sure if it will x-react with g.p). I used to use the Roche TUNEL
kit, but have not been happy with the consistency of the kit in the past
few years.
Good luck,
Melissa
-----Original Message-----
Dear Histonetter,
I would like hear some advice on good antibody against active Caspase-3.
Prefer it is working in different species.
Also, could anyone here recommend an apoptosis dectection kit (TUNEL
assay)?
We used to try the one from PerkinElmer.
I am working with guinea pig tissue.
Any input is greatly appreciated!
/Tenny
KI,stockholm
------------------------------
Message: 3
Date: Mon, 5 Dec 2005 20:38:11 +0100
From: "Emma JONES" <EJones <@t> Ventanamed.com>
Subject: [Histonet] re:MSH-2 and MLH-1
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F132EB22FDA6744E929F204E7B5B21BC943134 <@t> PROSPER.VENTANA.VENTANAMED.COM>
Content-Type: text/plain; charset="windows-1250"
Emma Jones
Hi Diana,
Have you asked your Ventana rep, for info on these antibodies.
They now sell both these primaries, pre-diluted and with recommended protocol.
MSH 2 Cat # 760-4265
MLH 1 Cat # 760-4264
Regards
Emma
Message: 12
Date: Fri, 2 Dec 2005 09:49:11 -0500
From: "Goodwin, Diana" <GoodwinD <@t> pahosp.com>
Subject: [Histonet] MSH-2 and MLH-1
To: "Histonet \(E-mail\)" <HistoNet <@t> Pathology.swmed.edu>
Message-ID:
<80CDD9C3FEEAFD4982B114C4A6DFD00E256F77 <@t> uphsmbx2.UPHS.PENNHEALTH.PRV>
Content-Type: text/plain; charset="iso-8859-1"
Greetings, Histonet.
I anyone running paraffin IHC with these Abs for human colo-rectal ca successfully on the Ventana Benchmark, and if so, which clone, vendor, dilution, etc.
Thanks in advance,
Diana Goodwin
Supervisor, Anatomic Pathology
Pennsylvania Hospital, Preston 655-C
ph: 215-829-6532
fax: 215-829-7564
e-mail: goodwind <@t> pahosp.com
--
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------------------------------
Message: 4
Date: Mon, 05 Dec 2005 21:27:23 +0000
From: "JEFF TATUM ZELIADT" <jzeliadt <@t> msn.com>
Subject: [Histonet] specific Ig detection
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY105-F18059CDC6A6EB27610F15FC4410 <@t> phx.gbl>
Content-Type: text/plain; format=flowed
I am a student taking an immunobiology course. I was wondering if someone
could help point me in the right direction. I have an unknown infections
agent, I know that it produces anaphlylaxis and will produce IgE and IgA.
If I isolate the IgE's from the serum I will get all IgE's even those not
specific to the bug. How can I seperate the two and get the specific
antigen? Can I use a protein which would be specific to the bug but how do
I know what protein?
------------------------------
Message: 5
Date: Mon, 05 Dec 2005 15:02:18 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] specific Ig detection
To: "JEFF TATUM ZELIADT" <jzeliadt <@t> msn.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20051205144633.01b2e820 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
You say "I know that it produces anaphlylaxis and will produce IgE and IgA
so isn't this called the Host response. In other words the host infected
by the "bug" responds to this by producing IgE (an allergic response as is
anaphylaxis along with IgA) I don't think the bug is producing these Ig's.
I suggest you try to get your "bug" identified via some microbiological
means, such as a diagnostic culturing protocol.
The serum of an animal infected with this infectious agent should contain
antiserum to the agent, hence antiserum i.e. antibodies to the bug should
be present in the serum of the infected host. So if you infected mouse
cells with the bug, then the serum of the mouse will contain mouse antiBug
antibodies - not necessarily IgA.
I guess I am confused on what you are trying to do here.
At 02:27 PM 12/5/2005, you wrote:
>I am a student taking an immunobiology course. I was wondering if someone
>could help point me in the right direction. I have an unknown infections
>agent,
>If I isolate the IgE's from the serum I will get all IgE's even those not
>specific to the bug. How can I seperate the two and get the specific
>antigen? Can I use a protein which would be specific to the bug but how
>do I know what protein?
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 6
Date: Tue, 06 Dec 2005 09:08:59 +0200
From: Mikael Niku <mikael.niku <@t> helsinki.fi>
Subject: [Histonet] Anti-DIG peroxidase problem
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4395390B.8040003 <@t> helsinki.fi>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear Histonetters,
I'm trying to set up tyramide amplification for my in situ
hybridizations with digoxigenin probes.
Unfortunately it seems as if the anti-DIG-POD by Roche doesn't work as
well as the anti-DIG-AP I'm normally using.
Basically, I get a weaker signal with anti-DIG-POD + tyramide
amplification than with unamplified anti-DIG-AP.
I have also tried biotinylated probes to avoid using anti-DIG-POD
altogether, but so far I haven't got any signal at all with them.
Any ideas, anyone?
With best regards,
Mikael Niku
--
////////////////////////////////////////////////////////////
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mitäkö mieltä olen länsimaisesta sivistyksestä?
Minusta se olisi erinomainen ajatus!
- Gandhi
////////////////////////////////////////////////////////////
------------------------------
Message: 7
Date: Tue, 6 Dec 2005 03:29:46 -0500
From: "Gus Mondragon" <gmondragon <@t> gsopath.com>
Subject: [Histonet] RE: Histonet Digest, Vol 25, Issue 5
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<03B63DE44B5C014D84F9A9D8A968B5174C4D3E <@t> lithium.corp.gsopath.com>
Content-Type: text/plain; charset="iso-8859-1"
A positive and a negative control is required by CLIA in all AFB stains (Leprosy and TB). A known positive control validates your staining procedure; a negative control eliminates the presence of artifact in your sections Gus Mondragon gmondragon <@t> gsopath.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Saturday, December 03, 2005 1:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 25, Issue 5
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Today's Topics:
1. Re: RE: Paraffin sections (Maria Mejia)
2. Extracellular matrix proteins fixation. Details
(Diego J. Rodr?guez Gil)
3. RE: Specail stains Negative controls (Smith, Jeffery D. (HSC))
4. Re: Specail stains Negative controls (Bryan Hewlett)
5. RE: Goat polymer (pruegg <@t> ihctech.net)
6. Knife holder (arunams)
7. RE: Question: Inventory (Favara, Cynthia (NIH/NIAID))
8. AW: [Histonet] Question: Inventory (Gudrun Lang)
----------------------------------------------------------------------
Message: 1
Date: Fri, 02 Dec 2005 10:16:05 -0800
From: Maria Mejia <maria <@t> ski.org>
Subject: Re: [Histonet] RE: Paraffin sections
To: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
Cc: histonet <@t> lists.utsouthwestern.edu, "C.M. van der Loos"
<c.m.vanderloos <@t> amc.uva.nl>, BMolinari <@t> heart.thi.tmc.edu
Message-ID: <43908F65.6010808 <@t> ski.org>
Content-Type: text/plain; charset=us-ascii; format=flowed
This type of experimenting would be interesting to check out especially
with
lower percentages of BSA. Andrea, I would agree that this overnight storage step probably is antigen dependent (Chris, that's what I meant by too much) and it would require a whole gamut testing of antibodies to see which
respond
positively and which would not...if one is inclined to do so.
Yours
Maria Bartola Mejia
Smith-Kettlewell Eye Research Institute
San Francisco, CA 94115
Email: maria <@t> ski.org
Phone: (415)-345-2185
Andrea T. Hooper wrote:
> Very interesting Chris! I have found sometimes this works and
> sometimes it doesn't and you get complete loss of staining -
> frustrating when one was just trying to save time to begin with!! I
> imagine it's antigen dependent but I have not had time to check.
>
> In my opinion Betsy, if you can avoid it (because you are just doing
> it to save time instead of setting up a workshop) then avoid it ... it
> simply isn't worth the risk IMHO.
>
>
>
> At 9:52 AM +0100 12/2/05, C.M. van der Loos wrote:
>
>> Betsy,
>>
>> For my NSH wet-workshops I tested the option of going up to endogenous
>> PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
>> Next day we started at the protein blocking step and then the rest.
>> Staining was as good as running the whole procedure in one day.
>>
>> Hope this info helps.
>>
>> Chris van der Loos, PhD
>> Dept. of Pathology
>> Academic Medical Center M2-230
>> Meibergdreef 9
>> NL-1105 AZ Amsterdam
>> The Netherlands
>
>
------------------------------
Message: 2
Date: Fri, 02 Dec 2005 13:55:04 -0500
From: "Diego J. Rodr?guez Gil" <Diego.RodriguezGil <@t> yale.edu>
Subject: [Histonet] Extracellular matrix proteins fixation. Details
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1133549704.43909888f401e <@t> webmail.med.yale.edu>
Content-Type: text/plain; charset=ISO-8859-1
Hi:
Thanks a lot to all of you who answer!. Since some of you asked, I am sending some more details.
I am working on mice (CD-1), and the molecules I am trying to stain are named Wnt (Wingless-Int). Antibodies are from R&D systems (most of them are goats), and there are no previous works reporting the use of these antibodies. I am only making the assumption that they work, based on the datasheet the company provides. I could see some very faint positive stain for some of them, but I am sure I losing some because of fixation. Besides that, I know they are expressed based on RT-PCR and in situ hybridization experiments.
Brief protocol is:
Perfusion with 4% PFA, and then overnight deep in 4% PFA at 4 C. Transfer to PBS, then 30% sucrose til they sink, freeze on OCT and then section on cryostat at -18C.
The antigen retrieval protocols that I tried were steaming (2, 5 and 10 min with sodium citrate) or pepsin in HCl. None of them gave me any better stainings.
Staining protocol: Wash in TBS and then add 2% BSA in TBS-T, for 30 min. Primary antibody (on 2% BSA) Over night .
Wash 3 x TBS-T.
Add secondary antibody on 2% BSA for an hour.
Wash 2 x TBS-T.
Wash TBS and mount with Crystal Mount.
Thanks again!
Best regards,
Diego.
--
Diego J. Rodríguez Gil
Postdoctoral Associate
Department of Neurosurgery
Yale University School of Medicine
PO Box 208082
333 Cedar St, FMB-430
New Haven CT, 06520-8082
U.S.A.
------------------------------
Message: 3
Date: Fri, 2 Dec 2005 13:23:19 -0600
From: "Smith, Jeffery D. \(HSC\)" <Jeffery-Smith <@t> ouhsc.edu>
Subject: RE: [Histonet] Specail stains Negative controls
To: "Grant, Debra, R" <drgrant <@t> cmh.edu>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EA80A7E0A540C44A8A339A3561E35C60063E262B <@t> PERCHERON.hsc.net.ou.edu>
Content-Type: text/plain; charset="iso-8859-1"
Debby,
Routine special stains only require a positive control as you are looking to see if the stain worked or not. Specimen is either positive or negative. No need for negative control. IHC uses both pos. and neg. controls because you are looking for varying intensities of staining as well as pos. or neg.
Best of Luck,
Jeff
________________________________
Hi Histonetters,
Does anyone run negative control slides with their GMS, AFB, and Gram special stains, and if so why?
Kind Regards,
Debby R. Grant HT(ASCP)
Histology Coordinator
The Children's Mercy Hospital & Clinics
2401 Gillham Rd.
Kansas City, Missouri 64108
lab (816)234-3827
fax (816) 802-1492
_______________________________________________
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------------------------------
Message: 4
Date: Fri, 2 Dec 2005 15:23:28 -0500
From: "Bryan Hewlett" <bhewlett <@t> cogeco.ca>
Subject: Re: [Histonet] Specail stains Negative controls
To: "Smith, Jeffery D. (HSC)" <Jeffery-Smith <@t> ouhsc.edu>, "Grant,
Debra, R" <drgrant <@t> cmh.edu>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002001c5f77e$786f0990$6400a8c0 <@t> mainbox>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Debby and Jeff,
Negative control slides are useful for GMS, Gram and AFB in order to
eliminate false positives due to contamination from the reagents, washing,
carry-over etc.
Regards,
Bryan
----- Original Message -----
From: "Smith, Jeffery D. (HSC)" <Jeffery-Smith <@t> ouhsc.edu>
To: "Grant, Debra, R" <drgrant <@t> cmh.edu>; <Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, December 02, 2005 2:23 PM
Subject: RE: [Histonet] Specail stains Negative controls
Debby,
Routine special stains only require a positive control as you are looking
to see if the stain worked or not. Specimen is either positive or negative.
No need for negative control. IHC uses both pos. and neg. controls because
you are looking for varying intensities of staining as well as pos. or neg.
Best of Luck,
Jeff
________________________________
Hi Histonetters,
Does anyone run negative control slides with their GMS, AFB, and Gram special stains, and if so why?
Kind Regards,
Debby R. Grant HT(ASCP)
Histology Coordinator
The Children's Mercy Hospital & Clinics
2401 Gillham Rd.
Kansas City, Missouri 64108
lab (816)234-3827
fax (816) 802-1492
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 5
Date: Fri, 2 Dec 2005 13:39:43 -0700
From: pruegg <@t> ihctech.net
Subject: RE: [Histonet] Goat polymer
To: "'Orr, Rebecca'" <ROrr <@t> enh.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200512022039.jB2KdkXX005415 <@t> pro12.abac.com>
Content-Type: text/plain; charset="us-ascii"
Becky,
Invitrogen is the company who makes the goat labeled polymer. As for blocking, I use serum free protein block before the primary and then before the rab. Anti-goat secondary and then again before the rabbit labeled polymer. If I still encounter non-specific staining I put some serum (from the host of the secondary, about 10%) in with the protein block. This method is very sensitive and you can bring up lots of non-specific BG so be careful to optimize your dilutions and times for the antibody and for the detection reagents. Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca
Sent: Friday, December 02, 2005 6:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Goat polymer
Hello everyone,
Just recently there was a posting on how to run a rabbit polymer with goat antibodies...or something to that nature, using an anti rabbit secondary and then the Dako polymer...could that person send me an email please? I had a quick question about blocking...
Also, does anyone have the name of the company that makes a goat polymer? I know there's a company out there...I've seen the ads but can't locate it...
Many thanks and have a great weekend!
Becky
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
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------------------------------
Message: 6
Date: Fri, 02 Dec 2005 14:04:38 -0800 (PST)
From: arunams <arunams <@t> interchange.ubc.ca>
Subject: [Histonet] Knife holder
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5641228.1133561078869.JavaMail.myubc2 <@t> portal9.itservices.ubc.ca>
Content-Type: text/plain; charset=us-ascii
Hi All
Does eny one know where to find a Tungston Carbide Knive holder for a Sorvall JB-4 Microtome. I really need one urgently. Thanks for your help Aruna Somasiri
------------------------------
Message: 7
Date: Fri, 2 Dec 2005 17:35:26 -0500
From: "Favara, Cynthia \(NIH/NIAID\)" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] Question: Inventory
To: "Luis Chiriboga" <Luis.Chiriboga <@t> med.nyu.edu>, "Histonet"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A985B7D45D355D4EB363288E717394375B8090 <@t> NIHCESMLBX5.nih.gov>
Content-Type: text/plain; charset="us-ascii"
I do not do high volume consistently but have some weeks where I am doing 200-300 stains per week. I do not register my reagents as the come into the lab. c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
Disclaimer:
The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives
-----Original Message-----
From: Luis Chiriboga [mailto:Luis.Chiriboga <@t> med.nyu.edu]
Sent: Thursday, December 01, 2005 11:14 AM
To: Histonet
Subject: [Histonet] Question: Inventory
Hi All
For those of you who are in high volume labs using ventana stainers, do you register all your reagents when they arrive? or do you register as you use? Thanks in advance Luis _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Sat, 3 Dec 2005 08:52:40 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Question: Inventory
To: "Histonetliste \(Histonetliste\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1133632800.18983.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
We register the bulk-reagents and prep-kits in the Ventana System just before use. The antibodies are registered, when they arrive, to have them on the list.
Gudrun Lang
Hi All
For those of you who are in high volume labs using ventana stainers, do you register all your reagents when they arrive? or do you register as you use? Thanks in advance Luis _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
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End of Histonet Digest, Vol 25, Issue 5
***************************************
------------------------------
Message: 8
Date: Tue, 6 Dec 2005 08:11:03 -0600
From: "Sheffield, Tiffany L" <Tiffany.L.Sheffield <@t> uth.tmc.edu>
Subject: [Histonet] Bone EM Question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CADC5BA4B34E5745906DBBB39A3D8D4F75A28C <@t> UTHEVS4.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Fellow Histonetters!
All you EM pro's, I have a question for you...What has been your
experience with bone and EM? What is the largest sample size you have
worked with (of course decaled)? I have been under the impression that
only very small pieces(or very young bone that is not very calcified
yet) that are very de-caled yield acceptable results. I am hearing
conflicting accounts as to sample size and whether it has to be decaled
or not. It is my understanding that it does have to be decaled and very
small if it has a shot of working. I have only worked with soft tissue
samples for EM. Any help would be greatly appreciated.
Thanks!
Tiffany Sheffield-Lopez, HT(ASCP)
Supervisor, Bone Histomorphometry & Biomaterials Lab
Department of Orthopaedic Surgery
UTHSC-Houston Medical School
6431 Fannin, Suite 6.144 MSB
Houston , TX 77030
713-500-6803 WK
713-500-0729 Fax
------------------------------
Message: 9
Date: Tue, 6 Dec 2005 11:03:34 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: [Histonet] FISH for HER2 testing
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B9101364056 <@t> MILW_MAIL1>
Content-Type: text/plain; charset="iso-8859-1"
All,
I have a pathologist who is looking for good turn around time on FISH HER2
testing. Their current turn around time is 3 weeks and the clinicians are
clamoring for something better. If anyone knows of a reference lab that can
do good FISH HER2 testing in a timely fashion, please let me know.
On a side note, has anyone experienced any problems with the Dako FDA
approved HercepTest kit when using it on breast core bx's? My Docs are not
confident using this kit (especially on our breast cores). Has anyone run
side by side comparisons between this kit and FISH HER2 testing?
If someone can suggest a good FISH HER2 reference lab, the second set of
questions are moot.
Thank-you In Advance,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
------------------------------
Message: 10
Date: Tue, 6 Dec 2005 18:06:29 +0100
From: "ole" <osteffe <@t> online.no>
Subject: [Histonet] Tropheryma whippleii
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000c01c5fa87$66d214a0$0100000a <@t> ole>
Content-Type: text/plain; charset="iso-8859-1"
Anyone know of a commercially available Anti-Tropheryma Wippleii antibody that works on FFPET?
Regards
Ole J Steffensen
Lab scientist
Dep of Pathology
Aalesund
Norway
------------------------------
Message: 11
Date: Tue, 6 Dec 2005 09:48:57 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] FISH for HER2 testing
To: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051206174857.93672.qmail <@t> web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Glen:
I cannot recommend you a reference lab because we used to do our own tests for the DAKO and the FISH (from VYSIS).
The results agreed and we used to run the DAKO tests on Thursdays and the FISH on Fridays on the cases requested from Thursday of one week to Wednesdays of the following.
Rene J.
"Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com> wrote:
All,
I have a pathologist who is looking for good turn around time on FISH HER2
testing. Their current turn around time is 3 weeks and the clinicians are
clamoring for something better. If anyone knows of a reference lab that can
do good FISH HER2 testing in a timely fashion, please let me know.
On a side note, has anyone experienced any problems with the Dako FDA
approved HercepTest kit when using it on breast core bx's? My Docs are not
confident using this kit (especially on our breast cores). Has anyone run
side by side comparisons between this kit and FISH HER2 testing?
If someone can suggest a good FISH HER2 reference lab, the second set of
questions are moot.
Thank-you In Advance,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
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End of Histonet Digest, Vol 25, Issue 8
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