[Histonet] Goat polymer
pruegg <@t> ihctech.net
pruegg <@t> ihctech.net
Fri Dec 2 14:39:43 CST 2005
Becky,
Invitrogen is the company who makes the goat labeled polymer. As for
blocking, I use serum free protein block before the primary and then before
the rab. Anti-goat secondary and then again before the rabbit labeled
polymer. If I still encounter non-specific staining I put some serum (from
the host of the secondary, about 10%) in with the protein block. This
method is very sensitive and you can bring up lots of non-specific BG so be
careful to optimize your dilutions and times for the antibody and for the
detection reagents.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca
Sent: Friday, December 02, 2005 6:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Goat polymer
Hello everyone,
Just recently there was a posting on how to run a rabbit polymer with
goat antibodies...or something to that nature, using an anti rabbit
secondary and then the Dako polymer...could that person send me an email
please? I had a quick question about blocking...
Also, does anyone have the name of the company that makes a goat
polymer? I know there's a company out there...I've seen the ads but
can't locate it...
Many thanks and have a great weekend!
Becky
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
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