[Histonet] (no subject)

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Dec 1 08:49:45 CST 2005


Hi Sionagh:
  Jore's fixative (1896):
  Water dist. ---100 mL + 40% formaldehyde--- 10 mL + sodium sulfate --- 2g + magnesium sulfate ---2 g + sodium chloride --- 1 g
  Klotz's: there are 3 formulas: 2 by Klotz & Coburn (1916) and 1 by Klotz & MacLachan (1915)
  The 1915 formula (original) is as follows:
  Water dist.---100 mL + 40% formaldehyde --- 0.5 mL + chloral hydrate --- 3 g + sodium sulfate --- 0.7 g + sodium bicarbonate --- 0.6 g + sodium chloride --- 0.55 g + potassium sulfate --- 0.06 g + potassium nitrate --- 1.2 g
  Rene J.
  

Sionagh Smith <Sionagh.Smith <@t> ed.ac.uk> wrote:
  Can anyone help me with a recipe for Jore's or Klotz fixative solutions? I 
have used both in the past for retaining colour and consistency of post 
mortem specimens for teaching veterinary students. I have been unable to 
find an actual recipe since someone else always prepared them! I wish I'd 
paid more attention now....

Any pointers would be much appreciated.

Sionagh Smith
Dept. of Veterinary Pathology
Royal (Dick) School of Veterinary Studies
Easter Bush Veterinary Centre
Easter Bush
Midlothian EH25 9RG

Tel 0131 650 8787



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