[Histonet] Re: Histonet Digest, Vol 21, Issue 41

Wed Aug 31 18:45:24 CDT 2005

Got the interview tomorrow

----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 01, 2005 2:30 AM
Subject: Histonet Digest, Vol 21, Issue 41


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> Today's Topics:
>
>   1. HT Certification ? (Dawson, Glen)
>   2. RE: HT Certification ? (Morken, Tim - Labvision)
>   3. RE: HT Certification ? (DDDeltour <@t> mar.med.navy.mil)
>   4. (no subject) (Matt Prideaux)
>   5. Re: Gulf Coast (Dana Marshall)
>   6. Re: Can PBS cause background (Brian Chelack)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 31 Aug 2005 10:39:17 -0500
> From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
> Subject: [Histonet] HT Certification ?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <D2401DE71F59D71184BC00D0B7479B91010B7FB4 <@t> MILW_MAIL1>
> Content-Type: text/plain; charset="iso-8859-1"
>
> All,
>
> I have an excellent lab assistant here that I believe would make an
> excellent histotech.  He happens to be from Mexico where he was an MLT (a 
> 3
> year degree program there).  I have his transpcripts from the college in
> Mexico but could anyone tell me who I could ask to see if or how many of 
> his
> credits could be applied to the ROUTE 2 for HT certification from the ASCP
> website?  This route states that with a year of experience in the histo 
> lab
> and 60 semester hours of appropriate academic credit, he could sit for the
> HT certification test.
>
> I would really like to be able to tell this individual if some of the
> courses he took in Mexico will be applicable so if anyone has any
> suggestions, please let me know.
>
> Thanx in Advance,
>
> Glen Dawson  BS, HT & QIHC (ASCP)
> IHC Manager
> Milwaukee, WI
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 31 Aug 2005 11:58:35 -0400
> From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
> Subject: RE: [Histonet] HT Certification ?
> To: "'Dawson, Glen'" <GDawson <@t> dynacaremilwaukee.com>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <0556BE8AC5551E4E8AF6BB9E42509BA20328D09F <@t> usca0082k08.labvision.apogent.com>
>
> Content-Type: text/plain
>
> Glenn, the ASCP recognizes Educational Credential Evaluators;  see at
> www.ece.org
>
> Tim Morken
> Lab Vision - Neomarkers
> www.labvision.com
>
> Free webhosting for US State Histotechnology Societies:
> http://www.labvisioncorp.com/demowebsite/index.cfm
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson, 
> Glen
> Sent: Wednesday, August 31, 2005 8:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] HT Certification ?
>
>
> All,
>
> I have an excellent lab assistant here that I believe would make an
> excellent histotech.  He happens to be from Mexico where he was an MLT (a 
> 3
> year degree program there).  I have his transpcripts from the college in
> Mexico but could anyone tell me who I could ask to see if or how many of 
> his
> credits could be applied to the ROUTE 2 for HT certification from the ASCP
> website?  This route states that with a year of experience in the histo 
> lab
> and 60 semester hours of appropriate academic credit, he could sit for the
> HT certification test.
>
> I would really like to be able to tell this individual if some of the
> courses he took in Mexico will be applicable so if anyone has any
> suggestions, please let me know.
>
> Thanx in Advance,
>
> Glen Dawson  BS, HT & QIHC (ASCP)
> IHC Manager
> Milwaukee, WI
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 31 Aug 2005 12:00:15 -0400
> From: DDDeltour <@t> mar.med.navy.mil
> Subject: RE: [Histonet] HT Certification ?
> To: GDawson <@t> dynacaremilwaukee.com, histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <080566D001A3D9459FFC0A391A646C9106C4AA3B <@t> marxchg03.mar.med.navy.mil>
> Content-Type: text/plain
>
> The best thing to do would be to contact ASCP directly. It says 
> "regionally
> accredited college/university". Accredited by whom though??
> Doug D
>
> -----Original Message-----
> From: Dawson, Glen [mailto:GDawson <@t> dynacaremilwaukee.com]
> Sent: Wednesday, August 31, 2005 11:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] HT Certification ?
>
> All,
>
> I have an excellent lab assistant here that I believe would make an
> excellent histotech.  He happens to be from Mexico where he was an MLT (a 
> 3
> year degree program there).  I have his transpcripts from the college in
> Mexico but could anyone tell me who I could ask to see if or how many of 
> his
> credits could be applied to the ROUTE 2 for HT certification from the ASCP
> website?  This route states that with a year of experience in the histo 
> lab
> and 60 semester hours of appropriate academic credit, he could sit for the
> HT certification test.
>
> I would really like to be able to tell this individual if some of the
> courses he took in Mexico will be applicable so if anyone has any
> suggestions, please let me know.
>
> Thanx in Advance,
>
> Glen Dawson  BS, HT & QIHC (ASCP)
> IHC Manager
> Milwaukee, WI
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 31 Aug 2005 17:08:09 +0100
> From: Matt Prideaux <M.Prideaux <@t> sheffield.ac.uk>
> Subject: [Histonet] (no subject)
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <1125504489.4315d5e9617de <@t> webmail.shef.ac.uk>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear Histonetters,
>
> A colleague wishes to store cytospun cell preparations in the freezer 
> before
> doing immunocytochemistry for CD31 and CD44. She would like to know 
> whether
> it's better to fix the slides before freezing or after thawing them prior 
> to
> staining. Also, which fixatives would you recommend?
>
> Matt
> -- 
> Matt Prideaux
> Academic Unit of Bone Biology
> Division of Clinical Sciences (South)
> D Floor (DU20) Medical School
> Henry Wellcome Laboratories for Medical Research
> University of Sheffield
> Beech Hill Road
> Sheffield
> S10 2RX
>
> Tel: 0114 271 3783
> Fax: 0114 271 1711
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 31 Aug 2005 11:34:35 -0500
> From: "Dana Marshall" <dmarsha3 <@t> utmem.edu>
> Subject: Re: [Histonet] Gulf Coast
> To: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001d01c5ae49$dfa84640$f623c084 <@t> DanaM>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> Good thinking Betsy.
> I second that.
> dm
> VAMC Memphis TN
>
> ----- Original Message ----- 
> From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, August 31, 2005 5:49 AM
> Subject: [Histonet] Gulf Coast
>
>
> My thoughts and prayers to all of you that are affected by Katrina.
>
>
>
> Betsy Molinari HT (ASCP)
>
> Texas Heart Institute
>
> Cardiovascular Pathology
>
> 6770 Bertner Ave.
>
> Houston,TX 77030
>
> 832-355-6524
>
> 832-355-6812 (fax)
>
>
>
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>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 31 Aug 2005 09:45:45 -0600
> From: Brian Chelack <BRIAN.CHELACK <@t> usask.ca>
> Subject: [Histonet] Re: Can PBS cause background
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4315D0A9.81D67496 <@t> sask.usask.ca>
> Content-Type: text/plain; charset=us-ascii
>
> Hello Carolyn;
>
> In the late 80's I did use an alternate procedure to
> inactivate endogenous peroxidases that included sodium
> borohydride. We were testing methods for antigens that were
> sensitive to H2O2-methanol. My memory is dim, but as I
> recall the procedure involved the immersion of the slides in
> 0.1% phenylhydrazine at 37C for 1 hour followed by immersion
> in 0.5 mg/ml NaBH4 for 10 minutes. In my experience bubbles
> under the sections were not a problem, but the treatment was
> tough on the sections. Phenylhydrazine is also not a very
> nice chemical to work with. If you are looking for a
> non-methonal method try using PBS + 2% H2O2 + 0.1% Na Azide.
>
> As far as PBS vs Tris based buffers is concerned, I believe
> the amino groups in the Tris can act to reduce nonspecific
> antibody binding. Over the years I have used both Tris and
> PBS based buffers and have seen differences in the staining
> intensity that is antibody specific. That is, certain
> antibodies do work slightly better with one buffer or the
> other. Ultimately the differences were not significant
> enough to warrant the use of one buffer over the other. We
> use PBS but Tris will work just fine.
>
> Brian Chelack
> Prairie Diagnostic Services
>
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 21, Issue 41
> ****************************************