[Histonet] HT certification

Wed Aug 31 12:38:38 CDT 2005

You can get information on this as well as certificate programs from
Harford Community College in Bel Air Maryland.  They offer long distance
programs via the internet. 

-----Original Message-----
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histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, August 31, 2005 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 21, Issue 41

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Today's Topics:

   1. HT Certification ? (Dawson, Glen)
   2. RE: HT Certification ? (Morken, Tim - Labvision)
   3. RE: HT Certification ? (DDDeltour <@t> mar.med.navy.mil)
   4. (no subject) (Matt Prideaux)
   5. Re: Gulf Coast (Dana Marshall)
   6. Re: Can PBS cause background (Brian Chelack)

----------------------------------------------------------------------

Message: 1
Date: Wed, 31 Aug 2005 10:39:17 -0500
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: [Histonet] HT Certification ?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B91010B7FB4 <@t> MILW_MAIL1>
Content-Type: text/plain;	charset="iso-8859-1"

All,

I have an excellent lab assistant here that I believe would make an
excellent histotech.  He happens to be from Mexico where he was an MLT
(a 3
year degree program there).  I have his transpcripts from the college in
Mexico but could anyone tell me who I could ask to see if or how many of
his
credits could be applied to the ROUTE 2 for HT certification from the
ASCP
website?  This route states that with a year of experience in the histo
lab
and 60 semester hours of appropriate academic credit, he could sit for
the
HT certification test.

I would really like to be able to tell this individual if some of the
courses he took in Mexico will be applicable so if anyone has any
suggestions, please let me know.

Thanx in Advance,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

------------------------------

Message: 2
Date: Wed, 31 Aug 2005 11:58:35 -0400
From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
Subject: RE: [Histonet] HT Certification ?
To: "'Dawson, Glen'" <GDawson <@t> dynacaremilwaukee.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:

<0556BE8AC5551E4E8AF6BB9E42509BA20328D09F <@t> usca0082k08.labvision.apogent.
com>

Content-Type: text/plain

Glenn, the ASCP recognizes Educational Credential Evaluators;  see at
www.ece.org

Tim Morken
Lab Vision - Neomarkers
www.labvision.com

Free webhosting for US State Histotechnology Societies:
http://www.labvisioncorp.com/demowebsite/index.cfm

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Wednesday, August 31, 2005 8:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HT Certification ?

All,

I have an excellent lab assistant here that I believe would make an
excellent histotech.  He happens to be from Mexico where he was an MLT
(a 3
year degree program there).  I have his transpcripts from the college in
Mexico but could anyone tell me who I could ask to see if or how many of
his
credits could be applied to the ROUTE 2 for HT certification from the
ASCP
website?  This route states that with a year of experience in the histo
lab
and 60 semester hours of appropriate academic credit, he could sit for
the
HT certification test.

I would really like to be able to tell this individual if some of the
courses he took in Mexico will be applicable so if anyone has any
suggestions, please let me know.

Thanx in Advance,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

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------------------------------

Message: 3
Date: Wed, 31 Aug 2005 12:00:15 -0400
From: DDDeltour <@t> mar.med.navy.mil
Subject: RE: [Histonet] HT Certification ?
To: GDawson <@t> dynacaremilwaukee.com, histonet <@t> lists.utsouthwestern.edu
Message-ID:

<080566D001A3D9459FFC0A391A646C9106C4AA3B <@t> marxchg03.mar.med.navy.mil>
Content-Type: text/plain

The best thing to do would be to contact ASCP directly. It says
"regionally
accredited college/university". Accredited by whom though??
Doug D

-----Original Message-----
From: Dawson, Glen [mailto:GDawson <@t> dynacaremilwaukee.com] 
Sent: Wednesday, August 31, 2005 11:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HT Certification ?

All,

I have an excellent lab assistant here that I believe would make an
excellent histotech.  He happens to be from Mexico where he was an MLT
(a 3
year degree program there).  I have his transpcripts from the college in
Mexico but could anyone tell me who I could ask to see if or how many of
his
credits could be applied to the ROUTE 2 for HT certification from the
ASCP
website?  This route states that with a year of experience in the histo
lab
and 60 semester hours of appropriate academic credit, he could sit for
the
HT certification test.

I would really like to be able to tell this individual if some of the
courses he took in Mexico will be applicable so if anyone has any
suggestions, please let me know.

Thanx in Advance,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

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------------------------------

Message: 4
Date: Wed, 31 Aug 2005 17:08:09 +0100
From: Matt Prideaux <M.Prideaux <@t> sheffield.ac.uk>
Subject: [Histonet] (no subject)
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1125504489.4315d5e9617de <@t> webmail.shef.ac.uk>
Content-Type: text/plain; charset=ISO-8859-1

Dear Histonetters,

A colleague wishes to store cytospun cell preparations in the freezer
before
doing immunocytochemistry for CD31 and CD44. She would like to know
whether
it's better to fix the slides before freezing or after thawing them
prior to
staining. Also, which fixatives would you recommend? 

Matt
-- 
Matt Prideaux
Academic Unit of Bone Biology
Division of Clinical Sciences (South)
D Floor (DU20) Medical School
Henry Wellcome Laboratories for Medical Research
University of Sheffield
Beech Hill Road
Sheffield 
S10 2RX

Tel: 0114 271 3783
Fax: 0114 271 1711

------------------------------

Message: 5
Date: Wed, 31 Aug 2005 11:34:35 -0500
From: "Dana Marshall" <dmarsha3 <@t> utmem.edu>
Subject: Re: [Histonet] Gulf Coast
To: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001d01c5ae49$dfa84640$f623c084 <@t> DanaM>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Good thinking Betsy.
I second that.
dm
VAMC Memphis TN

----- Original Message ----- 
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, August 31, 2005 5:49 AM
Subject: [Histonet] Gulf Coast

My thoughts and prayers to all of you that are affected by Katrina.  

Betsy Molinari HT (ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave.

Houston,TX 77030

832-355-6524

832-355-6812 (fax)

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------------------------------

Message: 6
Date: Wed, 31 Aug 2005 09:45:45 -0600
From: Brian Chelack <BRIAN.CHELACK <@t> usask.ca>
Subject: [Histonet] Re: Can PBS cause background
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4315D0A9.81D67496 <@t> sask.usask.ca>
Content-Type: text/plain; charset=us-ascii

Hello Carolyn;

In the late 80's I did use an alternate procedure to
inactivate endogenous peroxidases that included sodium
borohydride. We were testing methods for antigens that were
sensitive to H2O2-methanol. My memory is dim, but as I
recall the procedure involved the immersion of the slides in
0.1% phenylhydrazine at 37C for 1 hour followed by immersion
in 0.5 mg/ml NaBH4 for 10 minutes. In my experience bubbles
under the sections were not a problem, but the treatment was
tough on the sections. Phenylhydrazine is also not a very
nice chemical to work with. If you are looking for a
non-methonal method try using PBS + 2% H2O2 + 0.1% Na Azide.

As far as PBS vs Tris based buffers is concerned, I believe
the amino groups in the Tris can act to reduce nonspecific
antibody binding. Over the years I have used both Tris and
PBS based buffers and have seen differences in the staining
intensity that is antibody specific. That is, certain
antibodies do work slightly better with one buffer or the
other. Ultimately the differences were not significant
enough to warrant the use of one buffer over the other. We
use PBS but Tris will work just fine.

Brian Chelack
Prairie Diagnostic Services

------------------------------

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End of Histonet Digest, Vol 21, Issue 41
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