[Histonet] Cox-2 on human colon

Eeva, Mervi MEeva <@t> mednet.ucla.edu
Tue Aug 30 10:27:57 CDT 2005


So far we have used Goat Polyclonal sc-1745 from Santa Cruz w/ Vector ABC
kit  and Rabbit Polyclonal Cat. No.160126 from Cayman Chemical w/ Envision
kit.  First one stains too much:  background and all kind of cells. Later
one doesn't give strong enough signal.  Any experience of Transduction Labs
antibody ?
- Mervi
 


-----Original Message-----
From: Trajkovic, Dusko [mailto:dusko.trajkovic <@t> pfizer.com] 
Sent: Tuesday, August 30, 2005 7:10 AM
To: 'Eeva, Mervi'
Subject: RE: [Histonet] Cox-2 on human colon 

Which antibody are you using, or which ones have you tried?
Dusko

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eeva, Mervi
Sent: Friday, August 26, 2005 4:43 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cox-2 on human colon 

Hi,

 

Does anyone have suggestions about well working COX-2 antibody and protocol
on human colon tissue immunohistochemistry ? We have got it working well on
other tissues but colon is for some reason a problem: either too much
background or too little staining.   

 

Thanks,

-          Mervi

 

David Geffen School of Medicine at UCLA 
Gonda Center 

695 Charles E. Young Drive South 
Los Angeles, CA 90095-7088 
U.S.A. 

  

 


----------------------------------------------------------
IMPORTANT WARNING:  This email (and any attachments) is only intended for
the use of the person or entity to which it is addressed, and may contain
information that is privileged and confidential.  You, the recipient, are
obligated to maintain it in a safe, secure and confidential manner.
Unauthorized redisclosure or failure to maintain confidentiality may subject
you to federal and state penalties. If you are not the intended recipient,
please immediately notify us by return email, and delete this message from
your computer.
----------------------------------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



LEGAL NOTICE
Unless expressly stated otherwise, this message is confidential and may be
privileged. It is intended for the addressee(s) only. Access to this E-mail
by anyone else is unauthorized. If you are not an addressee, any disclosure
or copying of the contents of this E-mail or any action taken (or not taken)
in reliance on it is unauthorized and may be unlawful. If you are not an
addressee, please inform the sender immediately.

----------------------------------------------------------
IMPORTANT WARNING:  This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential.  You, the recipient, are obligated to maintain it in a safe, secure and confidential manner.  Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer.
----------------------------------------------------------




More information about the Histonet mailing list