[Histonet] Cox-2 on human colon

[Eeva, Mervi]

So far we have used Goat Polyclonal sc-1745 from Santa Cruz w/ Vector ABC
kit  and Rabbit Polyclonal Cat. No.160126 from Cayman Chemical w/ Envision
kit.  First one stains too much:  background and all kind of cells. Later
one doesn't give strong enough signal.  Any experience of Transduction Labs
antibody ?
- Mervi
 


-----Original Message-----
From: Trajkovic, Dusko [mailto:dusko.trajkovic <@t> pfizer.com] 
Sent: Tuesday, August 30, 2005 7:10 AM
To: 'Eeva, Mervi'
Subject: RE: [Histonet] Cox-2 on human colon 

Which antibody are you using, or which ones have you tried?
Dusko

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eeva, Mervi
Sent: Friday, August 26, 2005 4:43 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cox-2 on human colon 

Hi,

 

Does anyone have suggestions about well working COX-2 antibody and protocol
on human colon tissue immunohistochemistry ? We have got it working well on
other tissues but colon is for some reason a problem: either too much
background or too little staining.   

 

Thanks,

-          Mervi

 

David Geffen School of Medicine at UCLA 
Gonda Center 

695 Charles E. Young Drive South 
Los Angeles, CA 90095-7088 
U.S.A. 

  

 


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