[Histonet] trypsin verses chymotrypsin

[Goodpaster, Tracy A]

I would be interested in any comments on this topic as well!

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Elizabeth Chlipala
Sent: Mon 8/29/2005 1:37 PM
To: 'Histonet'
Subject: [Histonet] trypsin verses chymotrypsin



Hello everyone

Is there anyone out there that can explain the difference between
trypsin and chymotrypsin for retrieval in immunohistochemistry.  I'm
working out a protocol for aggrecan on cartilage and I have a protocol
that says they retrieved in chymotrypsin.  Any input would be
appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com

Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

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