[Histonet] Dried-out muscle biopsy

WWmn916 <@t> aol.com WWmn916 <@t> aol.com
Fri Aug 26 23:35:06 CDT 2005


Hello everyone,
 
I hoping someone can help me troubleshoot frozen muscle biopsies that are  
sometimes extremely difficult to cut because it's so dried out.  The muscle  
looks like it would be easy to cut, but ends up being difficult and sections  
hard to get.  What would "dry-out" muscle tissue?   Every now and  then, some 
muscle biopsies are so dried out that they seem like  powder coming off the 
cutting blade. Three things come to mind as  possible reasons: Specimen dried out 
shortly after procedure and transport,  during freezing the muscle, or the 
cryostat is too cold.  
 
Thanks for thoughts and responses!
 
Deb King
Sacramento, CA



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